{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:23:54Z","timestamp":1793813034551,"version":"4.6.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,7,3]],"date-time":"2012-07-03T00:00:00Z","timestamp":1341273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,2]]},"abstract":"\nDNMT3A<\/jats:styled-content>, a DNA<\/jats:styled-content> methyltransferase that functions for de novo<\/jats:italic> methylation, is important in development and many cellular processes related to tumorigenesis. Somatic mutations of \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> gene, including recurrent mutations in its Arg<\/jats:styled-content>\u2010882, were recently reported in acute myelogenous leukemia (AML<\/jats:styled-content>), strongly suggesting its role in development of AML<\/jats:styled-content>. To see whether \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> mutation occurs in other malignancies as well, we analyzed \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> in 916 cancer tissues from 401 hematologic malignancies (AML<\/jats:styled-content>, acute lymphoblastic leukemias (ALL<\/jats:styled-content>), multiple myelomas and lymphomas) and 515 carcinomas (lung, breast, prostate, colorectal and gastric carcinomas) using a single\u2010strand conformation polymorphism (SSCP<\/jats:styled-content>) assay. We identified \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> mutations, especially the Arg\u2010882 mutations, in adulthood AML<\/jats:styled-content> (9.4%). In addition, we found \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> mutations in pre\u2010B<\/jats:styled-content>\u2010ALL<\/jats:styled-content> and three lung cancers at lower frequencies. Allelic loss of \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> was frequently observed in most cancer types analyzed, including lymphomas (48.1%), gastric cancers (23.5%) and lung cancers (18.3%) irrespective of \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> mutation. Also, loss of DNMT3A<\/jats:styled-content> expression was common in lung cancers (46.4%), and was associated with the allelic loss. Our data indicate that \nDNMT3A<\/jats:italic>\n<\/jats:styled-content> gene is mutated mainly in AML<\/jats:styled-content>, but it occurs in other cancers, such as ALL<\/jats:styled-content> and lung cancer, despite the lower incidences. Also, the data suggest that DNMT3A<\/jats:styled-content> is altered in many cancer types by various ways, including somatic mutations, allelic loss and loss of expression that might play roles in tumorigenesis.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2012.02940.x","type":"journal-article","created":{"date-parts":[[2012,7,3]],"date-time":"2012-07-03T07:27:15Z","timestamp":1341300435000},"page":"85-94","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Mutational analysis of DNMT3A<\/i><\/scp> gene in acute leukemias and common solid cancers"],"prefix":"10.1111","volume":"121","author":[{"given":"Min S.","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Pathology College of Medicine The Catholic University of Korea Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoo R.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology College of Medicine The Catholic University of Korea Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam J.","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Pathology College of Medicine The Catholic University of Korea Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sug H.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology College of Medicine The Catholic University of Korea Seoul Korea"},{"name":"Integrative Research Center for Genome Polymorphism College of Medicine The Catholic University of Korea Seoul 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