{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T07:16:53Z","timestamp":1789888613427,"version":"4.0.1"},"reference-count":65,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,4,20]],"date-time":"2010-04-20T00:00:00Z","timestamp":1271721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Oral Implants Res"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective: <\/jats:bold> The aim of this human <jats:italic>in vivo<\/jats:italic> pilot study was to evaluate the efficacy of six antimicrobial agents on the surface decontamination of an oral biofilm attached to titanium implants.<\/jats:p><jats:p><jats:bold>Design: <\/jats:bold> For <jats:italic>in vivo<\/jats:italic> biofilm formation, we fixed titanium specimens to individual removable acrylic upper jaw splints (14 specimens in every splint), which were worn by four volunteers overnight for 12\u2003h. The specimens were then treated with different antimicrobial agents for 1\u2003min (Sodium hypochlorite, Hydrogen peroxide 3%, Chlorhexidingluconate 0.2%, Plax, Listerine, citric acid 40%). Afterwards, we quantified the total bacterial load and the viability of adhering bacteria by live or dead cell labelling in combination with fluorescence microscopy.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> The total bacterial load on the titanium surfaces was significantly higher after incubation in the control solution phosphate\u2010buffered saline (PBS) than after disinfection in sodium hypochlorite, hydrogen peroxide, chlorhexidine, Plax, Listerine, and citric acid. Furthermore, a significantly lower ratio between dead and total adhering bacteria (bactericidal effect) was found after incubation in control PBS, Plax mouth rinse, and citric acid than after incubation in sodium hypochlorite, hydrogen peroxide, chlorhexidine, and Listerine.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> All tested antiseptics seem to be able to reduce the total amount of microorganisms accumulating on titanium surfaces. Furthermore, sodium hypochlorite, hydrogen peroxide, chlorhexidine, and Listerine showed a significant bactericidal effect against adhering bacteria.<\/jats:p><jats:p> <jats:bold>To cite this article:<\/jats:bold>\n          \u2028Gosau M, Hahnel S, Schwarz F, Gerlach T, Reichert TE, B\u00fcrgers R. Effect of six different peri\u2010implantitis disinfection methods on <jats:italic>in vivo<\/jats:italic> human oral biofilm.\u2028<jats:italic>Clin. Oral Impl. Res<\/jats:italic>. <jats:bold>21<\/jats:bold>, 2010; 866\u2013872.\u2028doi: 10.1111\/j.1600\u20100501.2009.01908.x<\/jats:p>","DOI":"10.1111\/j.1600-0501.2009.01908.x","type":"journal-article","created":{"date-parts":[[2010,4,20]],"date-time":"2010-04-20T08:45:20Z","timestamp":1271753120000},"page":"866-872","source":"Crossref","is-referenced-by-count":128,"title":["Effect of six different peri\u2010implantitis disinfection methods on <i>in vivo<\/i> human oral biofilm"],"prefix":"10.1111","volume":"21","author":[{"given":"Martin","family":"Gosau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Hahnel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Schwarz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till","family":"Gerlach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten E.","family":"Reichert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"B\u00fcrgers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,7]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"190","article-title":"Effect of sodium hypochlorite solution on the subgingival microflora of juvenile periodontitis lesions","volume":"5","author":"Adcock J.E.","year":"1983","journal-title":"Pediatric Dentistry"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2008.02.014"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2002.290412.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028002168.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00048-2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.03.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016350802165614"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2008.07.011"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1995.66.11.962"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-008-0219-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01376.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1982.tb02049.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.2001.00971.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.10.942"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.070672"},{"key":"e_1_2_6_17_1","first-page":"327","article-title":"Supra\u2010 and subgingival biofilm formation on implant abutments with different surface characteristics","volume":"23","author":"Elter C.","year":"2008","journal-title":"The International Journal of Oral & Maxillofacial Implants"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2005.01137.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2007.01381.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(03)00015-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00074-3"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","unstructured":"Grusovin M.G. 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