{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:50:48Z","timestamp":1720745448925},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim: <\/jats:bold> Our study aimed to examine the molecular forms and gingival crevicular fluid (GCF) levels of laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain in patients with different periodontal disease, and compare the effects of <jats:italic>P.gingivalis<\/jats:italic> trypsin\u2010like proteinase on intact laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain species.<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> Eighteen patients with generalized aggressive periodontitis (G\u2010AgP), 29 patients with chronic periodontitis (CP), 20 with gingivitis and 20 periodontally healthy subjects were included. Probing depth, clinical attachment loss, presence of bleeding on probing and plaque were recorded. Molecular forms and GCF laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain levels and the effects of <jats:italic>P. gingivalis<\/jats:italic> trypsin\u2010like proteinase on intact laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain were analysed by computer\u2010quantitated Western immunoblotting.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> Laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain 40 and 70\u2003kDa fragments could be detected in all groups, in varying levels.The CP group had elevated GCF laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain fragment levels compared with the gingivitis and healthy groups (<jats:italic>p<\/jats:italic>&lt;0.008).The G\u2010AgP group had GCF laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain fragment levels similar to the gingivitis and healthy groups (<jats:italic>p<\/jats:italic>&gt;0.008). GCF laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain fragments differed clearly from the multiple lower molecular size fragments of <jats:italic>P.gingivalis<\/jats:italic> trypsin\u2010laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain proteinases.<\/jats:p><jats:p><jats:bold>Conclusion: <\/jats:bold> Increased GCF laminin\u20105 <jats:italic>\u03b3<\/jats:italic>2\u2010chain fragments in periodontitis sites with deep periodontal pocket suggest that these cleaved 40 and 70\u2003kDa fragments could reflect the extent of the inflammatory reaction in CP.<\/jats:p>","DOI":"10.1111\/j.1600-051x.2006.00933.x","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T12:00:03Z","timestamp":1149163203000},"page":"462-468","source":"Crossref","is-referenced-by-count":16,"title":["Gingival crevicular fluid laminin\u20105 <i>\u03b3<\/i>2\u2010chain levels in periodontal disease"],"prefix":"10.1111","volume":"33","author":[{"given":"G\u00fclnur","family":"Emingil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi","family":"Kuula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Piril\u00e4","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcl","family":"Atilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1985.tb00839.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00820.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02113.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411930040020401"},{"key":"e_1_2_8_7_1","first-page":"29","volume-title":"Crevicular Fluid Updated","author":"Cimasoni G.","year":"1983"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200006)218:2<213::AID-DVDY1>3.0.CO;2-R"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00780.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00584.x"},{"key":"e_1_2_8_11_1","first-page":"1394","article-title":"Effectiveness of adjunctive low\u2010dose doxycycline therapy on clinical parameters and gingival crevicular fluid laminin\u20105 \u03b32\u2010chain levels","volume":"75","author":"Emingil G.","year":"2004","journal-title":"Journal of Periodontology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2004.75.1.106"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.8.1025"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.8.1032"},{"key":"e_1_2_8_15_1","first-page":"87","article-title":"Phenotypic and Functional Analysis of Peripheral Blood Mononuclear Cells in Generalized Aggressive Periodontitis and Chronic Periodontitis Patients","volume":"3","author":"Emingil G.","year":"2001","journal-title":"Journal of the International Academy of Periodontology"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.32"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027005313.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.4.835"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5323.225"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260404.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027004262.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1996.67.3s.355"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.5s.521"},{"key":"e_1_2_8_24_1","first-page":"1267","article-title":"Degradation of basement membrane laminin by human neutrophil elastase and cathepsin G","volume":"136","author":"Heck L. 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