{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T16:46:27Z","timestamp":1768927587420,"version":"3.49.0"},"reference-count":53,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T00:00:00Z","timestamp":1315353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:sec><jats:label\/><jats:p>De Oliveira NFP, Andia DC, Planello AC, Pasetto S, Marques MR, Nociti FH, Line SRP, De Souza, AP. <jats:italic>TLR2<\/jats:italic> and <jats:italic>TLR4<\/jats:italic> gene promoter methylation status during chronic periodontitis. J Clin Periodontol 2011; 38: 975\u2013983. doi: 10.1111\/j.1600\u2010051X.2011.01765.x<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>\n              <jats:bold>Aim: <\/jats:bold> The objective of this study was to analyse the status of DNA methylation in the promoter region of the toll\u2010like receptor (<jats:italic>TLR<\/jats:italic>)<jats:italic>2<\/jats:italic> and <jats:italic>TLR4<\/jats:italic> genes in gingival tissue samples from healthy subjects, smokers and non\u2010smokers affected by chronic periodontitis.<\/jats:p><jats:p>\n              <jats:bold>Material and Methods: <\/jats:bold> Genomic DNA and total RNA were purified from gingival tissue using the TRIZOL reagent protocol. Genomic DNA was then digested by methylation\u2010sensitive restriction enzymes, amplified by polymerase chain reaction (PCR), electrophoresed on a 10% polyacrylamide gel and stained using SYBR Gold. Real\u2010time PCR was also performed to verify the transcript levels.<\/jats:p><jats:p>\n              <jats:bold>Results: <\/jats:bold> The CpG dinucleotides analysed were observed to be unmethylated in the majority of DNA samples of the three groups and statistical differences were not found among groups (<jats:italic>p<\/jats:italic>&gt;0.05). However, a trend towards methylation was observed in the <jats:italic>TLR2 Hha<\/jats:italic>I site in the samples of the periodontitis non\u2010smoker groups. In fact, the analysis of all CpG sites together shows which complete methylation is observed in the shortest level in the samples of periodontitis non\u2010smoker group. The analysis of transcript levels demonstrated no difference among groups (<jats:italic>p<\/jats:italic>&gt;0.05).<\/jats:p><jats:p>\n              <jats:bold>Conclusion: <\/jats:bold> The results demonstrated major unmethylation of the <jats:italic>TLR4<\/jats:italic> gene promoter in all groups. However, the results for the <jats:italic>TLR2<\/jats:italic> gene promoter are inconclusive; this gene was found as a mosaic of methylated and unmethylated DNA in the majority of samples of the three groups and we also observed a trend towards the DNA methylation of CpG sites recognized by the <jats:italic>Hha<\/jats:italic>I enzyme.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-051x.2011.01765.x","type":"journal-article","created":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T03:01:49Z","timestamp":1315450909000},"page":"975-983","source":"Crossref","is-referenced-by-count":53,"title":["<i>TLR2<\/i> and <i>TLR4<\/i> gene promoter methylation status during chronic periodontitis"],"prefix":"10.1111","volume":"38","author":[{"given":"Naila Francis Paulo","family":"De Oliveira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise 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