{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T01:28:25Z","timestamp":1752283705574},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T00:00:00Z","timestamp":1326844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Involvement of TLR2 in the pathophysiology of periodontitis has widely been discussed, but hitherto, no validated genetic associations were reported. Previous association studies lacked sufficient statistical power and adequate haplotype information to draw unambiguous conclusions. The aim of this study was to comprehensively investigate <jats:italic><jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content><\/jats:italic>2 linkage disequilibrium (<jats:styled-content style=\"fixed-case\">LD<\/jats:styled-content>) regions for their potential associations with periodontitis in two large analysis populations of aggressive (<jats:styled-content style=\"fixed-case\">AgP<\/jats:styled-content>) and chronic periodontitis (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>) of North West European descent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>The study population comprised 598 <jats:styled-content style=\"fixed-case\">AgP<\/jats:styled-content> patients, 914 <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> patients and 1804 healthy controls. Analysis of <jats:italic><jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content><\/jats:italic>2 <jats:styled-content style=\"fixed-case\">LD<\/jats:styled-content> regions was performed with haplotype tagging <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s (tagSNPs) using SNPlex and TaqMan genotyping assays. Genotypic, dominant, multiplicative, and recessive genetic models were tested. The genotypes were adjusted for the covariates smoking, diabetes, and gender. Resequencing was performed by Sanger technology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Upon covariate adjustment and correction for multiple testing, no tagSNPs showed significant associations with <jats:styled-content style=\"fixed-case\">AgP<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>. Targeted resequencing of exon 3 in 47 AgP cases identified carriership of two common and three rare variants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Common <jats:styled-content style=\"fixed-case\">LD<\/jats:styled-content> regions of <jats:italic><jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content><\/jats:italic>2 do not show genetic associations with periodontitis in the North West <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>uropean population. Resequencing of exon 3 could not identify disease\u2010associated rare variants in <jats:italic><jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content><\/jats:italic>2.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-051x.2011.01846.x","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T06:44:19Z","timestamp":1326869059000},"page":"315-322","source":"Crossref","is-referenced-by-count":7,"title":["Common genetic risk variants of <i><scp>TLR<\/scp><\/i>2 are not associated with periodontitis in large <scp>E<\/scp>uropean case\u2010control populations"],"prefix":"10.1111","volume":"39","author":[{"given":"Gesa M.","family":"Richter","sequence":"first","affiliation":[{"name":"Christian\u2010Albrechts\u2010University Institute for Clinical Molecular Biology Kiel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Graetz","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry 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Periodontology Academic Center for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"Folwaczny","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry and Periodontology University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Noack","sequence":"additional","affiliation":[{"name":"University Medical Center Carl Gustav Carus der Technischen Universit\u00e4t Dresden Zentrum f\u00fcr Zahn\u2010, Mund\u2010 und Kieferheilkunde, Poliklinik f\u00fcr Zahnerhaltung Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Eickholz","sequence":"additional","affiliation":[{"name":"Zentrum der Zahn\u2010, Mund\u2010 und Kieferheilkunde (Carolinum) der Johann Wolfgang Goethe\u2010Universit\u00e4t Frankfurt am Main Poliklinik f\u00fcr 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