{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:28:40Z","timestamp":1791268120540,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,6,13]],"date-time":"2012-06-13T00:00:00Z","timestamp":1339545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study investigated the periodontal regenerative potential of gingival margin\u2010derived multipotent postnatal stem\/progenitor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Periodontal defects were induced at six sites in eight miniature pigs in the premolar\/molar area (\u22124\u00a0weeks). Autologous cells isolated from the gingival margin were magnetically sorted using <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 antibodies and characterized flow cytometrically for the expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>31, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>117 and <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 surface markers. Colony formation and multilineage differentiation potential were tested. The cells were expanded and loaded on deproteinized bovine cancellous bone (<jats:styled-content style=\"fixed-case\">DBCB<\/jats:styled-content>) and Collagen scaffolds. Within every miniature pig, six periodontal defects were randomly treated with loaded\u2010<jats:styled-content style=\"fixed-case\">DBCB<\/jats:styled-content> (test group 1), unloaded\u2010<jats:styled-content style=\"fixed-case\">DBCB<\/jats:styled-content> (control group 1), loaded\u2010Collagen scaffolds (test group 2), unloaded\u2010Collagen scaffolds (control group 1), scaling and root planing (negative control 1) or left untreated (negative control 2). Differences in clinical attachment level (\u0394<jats:styled-content style=\"fixed-case\">CAL<\/jats:styled-content>), probing depth (\u0394<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>), gingival recession (\u0394<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>) and radiographic defect volume (\u0394<jats:styled-content style=\"fixed-case\">RDV<\/jats:styled-content>) between baseline and 12\u00a0weeks, as well as histological attachment level (<jats:styled-content style=\"fixed-case\">HAL<\/jats:styled-content>), junctional epithelium length (<jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content>) and connective tissue adhesion (<jats:styled-content style=\"fixed-case\">CTA<\/jats:styled-content>) after 12\u00a0weeks were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Isolated cells showed stem\/progenitor cell characteristics. Cell\u2010loaded scaffolds showed higher \u0394<jats:styled-content style=\"fixed-case\">CAL<\/jats:styled-content>, \u0394<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>, \u0394<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> HAL<\/jats:styled-content> and lower <jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CTA<\/jats:styled-content> compared with unloaded scaffolds and negative controls. The sort of scaffold had no significant influence on the measured outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gingival margin\u2010derived stem\/progenitor cells show significant periodontal regenerative potential.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-051x.2012.01904.x","type":"journal-article","created":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T14:10:54Z","timestamp":1337091054000},"page":"861-870","source":"Crossref","is-referenced-by-count":82,"title":["Periodontal regeneration employing gingival margin\u2010derived stem\/progenitor cells: an animal study"],"prefix":"10.1111","volume":"39","author":[{"given":"Karim M.","family":"Fawzy El\u2010Sayed","sequence":"first","affiliation":[{"name":"Clinic for Conservative Dentistry and Periodontology School of Dental Medicine Christian Albrechts University Kiel Germany"},{"name":"Oral Medicine and Periodontology Department Faculty of Oral and Dental Medicine Cairo University Cairo 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