{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T09:38:35Z","timestamp":1777109915543,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T00:00:00Z","timestamp":1169078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:p>To date, no <jats:italic>in vitro<\/jats:italic> test is suitable for routine diagnosis of contact allergy. The aim of our study was to establish improved <jats:italic>in vitro<\/jats:italic> test protocol for the detection of antigen\u2010specific responses of lymphocytes from patients with allergic contact dermatitis to nickel (Ni\u2010ACD). Blood leucocytes from 14 Ni\u2010ACD patients and 14 controls were cultured in the presence of \u2018cytokine cocktails\u2019 skewing lymphocytes towards \u2018type 1\u2019 [interferon\u2010\u03b3 (IFN\u2010<jats:bold>\u03b3<\/jats:bold>)\u2010secreting] or \u2018type 2\u2019 [interleukin (IL)\u20105 and IL\u201013\u2010secreting] phenotypes. The cocktails consisted of IL\u20107 and, respectively, either IL\u201012 or IL\u20104. Cell responses to nickel were measured with enzyme\u2010linked immunospot assay (ELISpot), enzyme\u2010linked immunosorbent assay (ELISA), and lymphocyte proliferation test (LPT). Significant differences between patients with Ni\u2010ACD and controls were found for the \u2018type 2\u2019 cytokines IL\u201013 and IL\u20105, with further increase of allergen\u2010specific responses occurring when cultures were supplemented with IL\u20107 and IL\u20104. No significant differences were found for IFN\u2010<jats:bold>\u03b3<\/jats:bold>. The best correlate to clinical diagnosis was LPT with \u2018type 2\u2019 skewing (<jats:italic>r<\/jats:italic>= 0.739, <jats:italic>P<\/jats:italic> &lt; 0.001), followed by IL\u201013 ELISpot with \u2018type 2\u2019 skewing (<jats:italic>r<\/jats:italic>= 0.654, <jats:italic>P<\/jats:italic> &lt; 0.001). The non\u2010radioactive method that correlated best with LPT was IL\u20102 ELISpot (<jats:italic>r<\/jats:italic>= 0.809, <jats:italic>P<\/jats:italic> &lt; 0.001). Overall, we conclude that combining ELISpot assay with proposed modifications of culture conditions improves detection of specific lymphocyte responses in contact allergy to nickel.<\/jats:p>","DOI":"10.1111\/j.1600-0536.2007.01045.x","type":"journal-article","created":{"date-parts":[[2007,1,18]],"date-time":"2007-01-18T19:10:15Z","timestamp":1169147415000},"page":"63-69","source":"Crossref","is-referenced-by-count":50,"title":["Allergic contact dermatitis to nickel: modified <i>in vitro<\/i> test protocols for better detection of allergen\u2010specific response"],"prefix":"10.1111","volume":"56","author":[{"given":"Radoslaw","family":"Spiewak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heleen","family":"Moed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitta Mary E.","family":"Von 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