{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T01:28:28Z","timestamp":1721957308688},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T00:00:00Z","timestamp":1157500800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold>Tuberous sclerosis complex (TSC) is associated with mutations in two likely tumor\u2010suppressor genes (<jats:italic>TSC1<\/jats:italic>and<jats:italic>TSC2<\/jats:italic>) and characterized by the development of tumor\u2010like growths (angiofibromas) in a variety of tissues and organs, particularly brain and skin.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>Employing a DNA\u2010microarray assay, able to detect mRNA production from 1176 different basic genes, we analyzed the gene\u2010expression levels in a cutaneous hamartoma sample from a TSC patient. Altered gene expressions detected by microarray technology were further checked by quantitative reverse\u2010transcriptase polymerase chain reaction (RT\u2010PCR) in the same material and in cutaneous hamartoma samples obtained from five other TSC patients.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>The results obtained by the microarray technology in one hamartoma specimen, confirmed by the RT\u2010PCR results obtained in the same material and in five other hamartoma specimens, demonstrated that TSC\u2010related angiofibromas exhibit significant mRNA overexpression of two genes, represented by<jats:italic>MLH\u20101<\/jats:italic>and<jats:italic>psoriasin<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>The overexpression of<jats:italic>MLH\u20101<\/jats:italic>, which codes for a DNA mismatch repair protein, and<jats:italic>psoriasin<\/jats:italic>, which codes for a specific chemoattractant factor for CD4<jats:sup>+<\/jats:sup>T cells, implicated in the pathogenesis of inflammatory skin disease, and expressed in excess during abnormal pathways of cell growth, may shed light on the pathogenesis of the proliferative skin lesion.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2006.00483.x","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T14:11:10Z","timestamp":1157551870000},"page":"608-613","source":"Crossref","is-referenced-by-count":5,"title":["Overexpression of<i>MLH\u20101<\/i>and<i>psoriasin<\/i>genes in cutaneous angiofibromas from tuberous sclerosis complex patients"],"prefix":"10.1111","volume":"33","author":[{"given":"Michelangelo","family":"La 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