{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:55:19Z","timestamp":1761058519523},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T00:00:00Z","timestamp":1156896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Diffuse alopecia occurs in almost 7% of HIV\u20101\u2010infected patients. Telogen effluvium is the main pathogenic mechanism involved. Apoptotic keratinocytes in the outer root sheath at bulge level was described as the most characteristic histopathologic finding of this kind of hair loss.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> A case\u2013control study was conducted to investigate the occurrence of apoptosis of follicular stem cells at the bulge in diffuse alopecia of HIV\u20101 infection. We applied a double\u2010staining procedure to transverse scalp sections from 15 HIV\u20101\u2010infected patients and 12 controls, with the monoclonal antibody anticytokeratin 19 as stem cell marker and TUNEL technique to identify apoptosis.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Eighty percent of cases and 25% of controls presented at least one double\u2010stained follicle. The proportion of positive follicles per section was 48% (\u00b17%) for cases and 26% (\u00b113%) for controls.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our study demonstrated that diffuse alopecia related to HIV\u20101 infection represents a hair cycle disturbance and that part of the follicular stem cell population become apoptotic in a higher proportion than normal subjects. We found no cytotoxic folliculitis. Owing to its cell\u2010cycle interaction and caspase\u2010induction capacities, we propose HIV\u20101 viral protein R as a possible follicular stem cell apoptosis inductor.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2006.00517.x","type":"journal-article","created":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T19:43:32Z","timestamp":1156967012000},"page":"667-671","source":"Crossref","is-referenced-by-count":14,"title":["Stem cell apoptosis in HIV\u20101 alopecia"],"prefix":"10.1111","volume":"33","author":[{"given":"Carlos Baptista","family":"Barcaui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Maria","family":"Gon\u00e7alves da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00edrian N.","family":"Sotto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Genser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/00128071-200203010-00006"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.01649.x"},{"key":"e_1_2_6_4_2","first-page":"292","article-title":"Cutaneous side effects induced by indinavir","volume":"10","author":"Calista D","year":"2000","journal-title":"Eur J Dermatol"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(96)90835-X"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000900302"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsp.5640228"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90696-C"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2004.07209008.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.19644.x"},{"key":"e_1_2_6_11_2","first-page":"411","article-title":"Parafollicular bulges, but not hair bulb keratinocytes, are attacked in graft\u2010versus\u2010host disease of human skin","volume":"14","author":"Sale GE","year":"1994","journal-title":"Bone Marrow Transplant"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.1991.tb01541.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1001\/archderm.1984.01650400031009","article-title":"Transverse microscopy anatomy of the human scalp. A basis for a morphometric approach to disorders of the hair follicle","volume":"120","author":"Headington JT","year":"1984","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12392730"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.5.1017"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/0022-202X(89)90186-3","article-title":"Keratin 19: predicted aminoacid sequence and broad tissue distribution suggest it evolved from keratinocyte keratins","volume":"92","author":"Stasiak PC","year":"1989","journal-title":"J Invest Dermatol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsp.5640233"},{"key":"e_1_2_6_18_2","first-page":"18","article-title":"Alopecia: histologic diagnosis by transverse sections","volume":"2","author":"Templeton SF","year":"1996","journal-title":"Semin Diagn Pathol"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199908193410818"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.136.8.1064"},{"key":"e_1_2_6_21_2","first-page":"6","article-title":"Indinavir use: associated reversible hair loss and mood disturbance","volume":"474","author":"Harry TC","year":"2000","journal-title":"Int J STD AIDS"},{"key":"e_1_2_6_22_2","first-page":"96","article-title":"Generalized hair loss induced by indinavir plus ritonavir therapy","volume":"1695","author":"Ginarte M","year":"2002","journal-title":"AIDS"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81874-7"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.6.1675"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.61.5.559"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90057-8"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.156"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.9.2951"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.4.F11"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4992"},{"key":"e_1_2_6_31_2","first-page":"11","article-title":"Human Immuno\u2010deficiency virus type 1 Vpr induces apoptosis through caspase activation","volume":"3105","author":"Stewart SA","year":"2000","journal-title":"J Virol"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.3109\/13550289809114529"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0560.2006.00517.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0560.2006.00517.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T20:18:40Z","timestamp":1697055520000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0560.2006.00517.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":31,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1111\/j.1600-0560.2006.00517.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0560.2006.00517.x","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]}}}