{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T07:16:35Z","timestamp":1773040595117,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T00:00:00Z","timestamp":1156896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Broad\u2010spectrum fungal stains are used to detect fungal organisms, but narrow\u2010spectrum stains can assist in fungal differential diagnosis. These stains include mucicarmine and Fontana\u2013Masson (FM) for <jats:italic>Cryptococcus<\/jats:italic>, Alcian Blue for <jats:italic>Cryptococcus<\/jats:italic> and <jats:italic>Blastomyces<\/jats:italic>, Congo Red for <jats:italic>Blastomyces<\/jats:italic> and <jats:italic>Coccidioides<\/jats:italic>, and Ziehl\u2013Neelsen for some examples of <jats:italic>Blastomyces<\/jats:italic> and <jats:italic>Histoplasma<\/jats:italic>. <jats:italic>Pityrosporum<\/jats:italic> is increasingly being recognized as a pathogen capable of significant cutaneous and systemic infections, but the narrow\u2010spectrum staining pattern of <jats:italic>Pityrosporum<\/jats:italic> has not yet been systematically studied and reported.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Paraffin\u2010embedded archival tissue from 10 skin biopsies containing <jats:italic>Pityrosporum<\/jats:italic> was stained with Alcian Blue, Congo Red, Ziehl\u2013Neelsen acid\u2010fast stain, and FM stain. In addition to the 60\u2010min staining time, a modified FM stain with 15\u2010, 30\u2010, and 45\u2010min staining times was also performed.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> All cases stained positive with Alcian Blue, Congo Red, and FM stains and negative with the Ziehl\u2013Neelsen stain. The modified FM stain with the 15\u2010min staining time showed significant staining in more than 50% of the cases.<\/jats:p><jats:p><jats:bold>Discussion:\u2002<\/jats:bold> The narrow\u2010spectrum staining pattern of <jats:italic>Pityrosporum<\/jats:italic> distinguishes it from <jats:italic>Cryptococcus<\/jats:italic>, <jats:italic>Blastomyces<\/jats:italic>, <jats:italic>Candida<\/jats:italic>, <jats:italic>Histoplasma<\/jats:italic>, and <jats:italic>Coccidioides<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2006.00537.x","type":"journal-article","created":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T19:43:32Z","timestamp":1156967012000},"page":"731-734","source":"Crossref","is-referenced-by-count":10,"title":["Narrow\u2010spectrum staining pattern of <i>Pityrosporum<\/i>*"],"prefix":"10.1111","volume":"33","author":[{"given":"Ranjitha","family":"Veerappan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lou Ellen","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Sosinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George A.","family":"Youngberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,3]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(82)80267-0"},{"key":"e_1_2_5_3_2","article-title":"The coloration of acidic carbohydrates of bacteria and fungi in tissue sections with special reference to capsules of Cryptococcus neoformans, pneumococci, and staphylococci","volume":"32","author":"Mowry RW","year":"1956","journal-title":"Am J Pathol"},{"key":"e_1_2_5_4_2","article-title":"Production of diagnostic pigment by phenoloxidase activity of Cryptococcus neoformans","volume":"24","author":"Shaw CE","year":"1972","journal-title":"Appl Microbiol"},{"key":"e_1_2_5_5_2","volume-title":"Pathology of tropical extraordinary diseases: an atlas","author":"Binford CR","year":"1976"},{"key":"e_1_2_5_6_2","article-title":"Atypical systemic fungal infections, case two: diffuse small\u2010form pulmonary B dermatitidis with concomitant alveolar cell carcinoma","volume":"72","author":"Laurain AR","year":"1979","journal-title":"South Med J"},{"key":"e_1_2_5_7_2","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.9.3.382","article-title":"Detection of infection or infectious agents by use of cytologic and histologic stains","volume":"9","author":"Woods GL","year":"1996","journal-title":"Clin Microbiol Rev"},{"key":"e_1_2_5_8_2","first-page":"159","volume-title":"Theory and practice of histotechnology","author":"Sheehan DC","year":"1980"},{"key":"e_1_2_5_9_2","article-title":"New, special stain for histopathological diagnosis of cryptococcosis","volume":"13","author":"Kwon\u2010Chung KJ","year":"1981","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_10_2","article-title":"Acid\u2010fastness of fungi in blastomycosis and histoplasmosis","volume":"106","author":"Wages DS","year":"1982","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_5_11_2","first-page":"1","volume-title":"Pathologic diagnosis of fungal infections.","author":"Chandler FW","year":"1987"},{"key":"e_1_2_5_12_2","article-title":"Advantage of. 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