{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T17:43:22Z","timestamp":1743011002981},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T00:00:00Z","timestamp":1162252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold>Nickel can induce cellular and nuclear damages responsible for chronic diseases, like allergic contact dermatitis (ACD). We previously showed that matrix metalloproteinase\u20102 (MMP\u20102) gene expression was induced by nickel in nontumorigenic human keratinocytes cell line (HaCat).<\/jats:p><jats:p><jats:bold>Objective:\u2002<\/jats:bold>To investigate the signal transduction pathways involved in gelatinolytic activity induced in HaCat under nickel stimulation.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>We analyzed the involvement of protein kinase A (PKA), protein kinase C (PKC), tyrosine kinase (PTK), nuclear factor\u2010kB (NF\u2010kB) and activator protein\u20101 (AP\u20101) using specific inhibitors (H89, calphostin C, genistein, carpain and curcumin) by electrophoretic mobility shift assay, reverse transcription\u2010polymerase chain reaction and gelatin zymography.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>Our results indicate that nickel\u2010induced MMP\u20102 production was inhibited with PTK, PKC and AP\u20101 specific inhibitors. Moreover, both PKA and NF\u2010kB were not involved in nickel pathway.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>Using HaCat, we showed that curcumin and genistein can revert nickel\u2010induced MMP\u20102 upregulation. Whether the use of PTK and AP\u20101 inhibitors has therapeutic ramifications in the management of ACD remains to be investigated.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2006.00647.x","type":"journal-article","created":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T12:45:32Z","timestamp":1162298732000},"page":"441-447","source":"Crossref","is-referenced-by-count":14,"title":["Analysis of the signal transduction pathway of nickel\u2010induced matrix metalloproteinase\u20102 expression in the human keratinocytes<i>in vitro<\/i>: preliminary findings"],"prefix":"10.1111","volume":"34","author":[{"given":"Brunella","family":"Perfetto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Lamberti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria 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