{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:35:57Z","timestamp":1770824157639,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T00:00:00Z","timestamp":1166054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p>The histopathologic distinction between pigmented actinic keratosis (PAK) and atypical junctional melanocytic proliferations (AJMP) is a common problem, and it is one with meaningful clinical significance. Previous publications have suggested that Melanocyte Antigen Related to T\u2010cells\u20101 (MART\u20101) \u2013 a melanocytic marker related to host immune response \u2013 was not useful in making this interpretative separation. To revisit that assertion, the authors selected 68 specimens that concerned the diagnosis of PAK vs. AJMP. The degree of morphologic difficulty attached to each case was rated semiquantitatively using a three\u2010tiered scale, and interpretative problems were caused by cytologic similarity between atypical keratinocytes and aberrant melanocytes, obscuring lichenoid inflammation, subepidermal fibrosis, and an absence of clearly defined cell nests at the dermoepidermal junction. Each biopsy sample was immunostained for MART\u20101 (using antibody clone A103) with azure\u2010B counterstaining; the principal criterion for a diagnosis of AJMP was that of confluent cellular positivity over at least 1 high\u2010power (\u00d7400) microscopic field, in conjunction with nested cell growth. The specimens were then re\u2010examined diagnostically. Immunostaining definitely improved interpretative certitude in 65 examples (96% effectiveness); the final diagnosis was that of PAK for 21 lesions and AJMP for 47. Three specimens \u2013 all of which represented AJMP \u2013 did not benefit by MART\u20101 staining. It is concluded that MART\u20101 immunostaining with azure\u2010B counterstaining is a useful adjunct in the interpretation of problematic intra\u2010epidermal pigmented lesions.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2006.00673.x","type":"journal-article","created":{"date-parts":[[2006,12,15]],"date-time":"2006-12-15T00:58:41Z","timestamp":1166144321000},"page":"601-605","source":"Crossref","is-referenced-by-count":29,"title":["Immunostaining for MART\u20101 in the interpretation of problematic intra\u2010epidermal pigmented lesions"],"prefix":"10.1111","volume":"34","author":[{"given":"Katy L.","family":"Wiltz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hina","family":"Qureshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Patterson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel C.","family":"Mayes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R.","family":"Wick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,14]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"crossref","first-page":"762","DOI":"10.4049\/jimmunol.154.2.762","article-title":"Generation of tumor\u2010specific CTLs from melanoma patients by using peripheral blood stimulated with allogeneic melanoma tumor cell lines. 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