{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:26:26Z","timestamp":1790814386688,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,3,10]],"date-time":"2008-03-10T00:00:00Z","timestamp":1205107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Lichen planus is an inflammatory dermatosis involving either skin and\/or mucosal epithelial surfaces. A cell\u2010mediated cytotoxicity response is the main suspected mechanism of this dermatosis. Granzyme B and granulysin are components of the cytoplasmic granules of cytotoxic T lymphocytes and natural killers. They are involved in cell\u2010mediated apoptosis. This work studies the possible implication of granzyme B and granulysin in the cell\u2010mediated cytotoxicity response in lichen planus.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>  <jats:italic>In situ<\/jats:italic> expression of granzyme B and granulysin was studied by real\u2010time reverse transcriptase polymerase chain reaction in 15 biopsies of lichen planus. The distribution and the phenotype of the inflammatory infiltrate and the expression of granzyme B were studied by immunohistochemistry in seven other biopsies of lichen planus.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Granzyme B and granulysin mRNA expression was one to two hundred times greater than in biopsies of normal skin. Immunohistochemical study revealed that the lymphohistiocytic infiltrate consisted mainly of CD4+ and CD8+ lymphocytes. Granzyme B+ cells were observed close to apoptotic keratinocytes.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our results suggest a central role for cell\u2010mediated cytotoxicity by the granule exocytosis pathway probably because of auto\u2010cytotoxic T\u2010cell clones in the pathogenesis of lichen planus.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2007.00892.x","type":"journal-article","created":{"date-parts":[[2008,3,11]],"date-time":"2008-03-11T03:16:07Z","timestamp":1205205367000},"page":"630-634","source":"Crossref","is-referenced-by-count":39,"title":["Involvement of granzyme B and granulysin in the cytotoxic response in lichen planus"],"prefix":"10.1111","volume":"35","author":[{"given":"M.","family":"Ammar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mokni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Boubaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel","family":"El Gaied","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ben 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