{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T10:33:07Z","timestamp":1723199587607},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,3,6]],"date-time":"2011-03-06T00:00:00Z","timestamp":1299369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p> <jats:bold>Background:<\/jats:bold> Alpha v integrins are receptors for many extracellular matrix (ECM) protein ligands, including latent transforming growth factor betas (TGF\u03b2s). Various studies in mice have shown that ablation of genes encoding \u03b1v integrin or TGF\u03b2 signaling pathway components leads to spontaneous squamous cell carcinomas (SCCs) in the conjunctiva and periocular skin. Here, we have analyzed patterns of \u03b1v integrin protein expression and TGF\u03b2 signaling in human eyelid and periorbital SCC samples.<\/jats:p><jats:p> <jats:bold>Methods:<\/jats:bold> An anti\u2010\u03b1v integrin antibody was used to immunostain 19 eyelid and periorbital SCC samples. Additionally, tissue lysates from resected normal eyelid and SCC samples were analyzed by immunoblotting for \u03b1v integrin protein. Tumor sections were also immunostained with an antibody directed against Smad2, an intracellular signaling protein that is phosphorylated by TGF\u03b2 receptors.<\/jats:p><jats:p> <jats:bold>Results:<\/jats:bold> Alpha v integrin protein was highly expressed in the invasive and less\u2010differentiated components of human SCCs. Lower levels of \u03b1v integrin protein were detected in more differentiated components of tumors, as well as in SCC <jats:italic>in situ<\/jats:italic>. Patterns of phosphorylated Smad2 immunoreactivity correlated with levels \u03b1v integrin expression.<\/jats:p><jats:p> <jats:bold>Conclusions:<\/jats:bold> Alpha v integrin was expressed at robust levels in tumor cells representing less differentiated, more invasive components of SCC; by contrast, well\u2010differentiated cells as well as SCC <jats:italic>in situ<\/jats:italic> expressed low levels of \u03b1v integrin protein.<\/jats:p><jats:p>Hsu A, Esmaeli B, Hayek B, Hossain MG, Shinder R, Lazar AJ, McCarty JH. Analysis of \u03b1v integrin protein expression in human eyelid and periorbital squamous cell carcinomas.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2011.01687.x","type":"journal-article","created":{"date-parts":[[2011,3,7]],"date-time":"2011-03-07T02:06:27Z","timestamp":1299463587000},"page":"570-575","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of \u03b1v integrin protein expression in human eyelid and periorbital squamous cell carcinomas"],"prefix":"10.1111","volume":"38","author":[{"given":"Adam","family":"Hsu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bita","family":"Esmaeli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brent","family":"Hayek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad G.","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Shinder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander J.","family":"Lazar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph H.","family":"McCarty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,3,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2748"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf399"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.3.729"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.5.1357"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.2.559"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1817"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-1640"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20118"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00725"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.070700"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.199"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002010203"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.07.001"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00008390-200402000-00005"},{"key":"e_1_2_6_16_2","first-page":"4073.","article-title":"Alphav integrin expression is a novel marker of poor prognosis in advanced\u2010stage ovarian carcinoma.","volume":"7","author":"Goldberg I","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2168"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-1847"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2353"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-007-0138-9"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1122"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011947301849"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2556"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/07357900701522638"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0560.2011.01687.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0560.2011.01687.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T15:08:10Z","timestamp":1698678490000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0560.2011.01687.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,6]]},"references-count":23,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1111\/j.1600-0560.2011.01687.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0560.2011.01687.x","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,6]]}}}