{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:13:14Z","timestamp":1765231994479},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T00:00:00Z","timestamp":1329350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The diagnosis of early mycosis fungoides (MF) is a big challenge to dermatologists and dermatopathologists because it lacks specific clinicopathologic features.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Fifty\u2010two paraffin\u2010embedded skin samples from 50 patients, including 31 with suspected MF, 10 with typical MF and 9 with benign inflammatory dermatosis (BID), were obtained from our archives. DNA was extracted both by traditional phenol\u2010chloroform method and by the laser\u2010capture microdissection (LCM)\u2010proteinase K approach. The T<jats:sub>VG<\/jats:sub>\/T<jats:sub>JG<\/jats:sub>, V<jats:sub>2\u20135<\/jats:sub>\/V<jats:sub>8\u201312<\/jats:sub>\/JGT<jats:sub>1<\/jats:sub> and BIOMED\u20102\u2010TCR\u2010\u03b3 primers were used to assess TCR\u2010\u03b3 monoclonal rearrangement as measured by polymerase chain reaction (PCR).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In the suspected MF group, clonal TCR\u2010\u03b3 gene rearrangements were detected in 11\/31 cases (35.5%) by phenol\u2010chloroform DNA extraction and in 25\/31 cases (80.7%) by LCM\u2010proteinase K extraction (p &lt; 0.05). While T\u2010cell clonality was detected in 8\/10 cases (80%) by the phenol\u2010chloroform method and 10\/10 cases (100%) by LCM (p &gt; 0.05) in the typical MF group, no TCR\u2010\u03b3 monoclonal rearrangement was detected in the BID group.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The strategy of multiple PCR\/heteroduplex analysis for TCR\u2010\u03b3 gene rearrangement combined with LCM increases the detection rate of clonal TCR\u2010\u03b3 gene rearrangement in early MF cases and could provide strong evidence to confirm the diagnosis of early MF.<\/jats:p><jats:p>Yang H, Xu C, Tang Y, Wan C, Liu W, Wang L. The significance of multiplex PCR\/heteroduplex analysis\u2010based <jats:italic>TCR<\/jats:italic>\u2010\u03b3 gene rearrangement combined with laser\u2010capture microdissection in the diagnosis of early mycosis fungoides.<\/jats:p>","DOI":"10.1111\/j.1600-0560.2011.01842.x","type":"journal-article","created":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T08:58:05Z","timestamp":1329382685000},"page":"337-346","source":"Crossref","is-referenced-by-count":6,"title":["The significance of multiplex PCR\/heteroduplex analysis\u2010based <i>TCR\u2010\u03b3<\/i> gene rearrangement combined with laser\u2010capture microdissection in the diagnosis of early mycosis 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