{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:43:54Z","timestamp":1770835434933,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T00:00:00Z","timestamp":1342656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"Background<\/jats:title>Spindle cell melanoma represents a rare but distinct subset of melanoma, and its genomic spectrum has not been fully defined.<\/jats:p><\/jats:sec>Methods<\/jats:title>We searched our institutional database for patients with a diagnosis of pure spindle cell\u2010type melanoma whose tumors had been analyzed for BRAF<\/jats:styled-content><\/jats:italic>, NRAS<\/jats:styled-content><\/jats:italic>, and KIT<\/jats:styled-content><\/jats:italic> mutations using pyrosequencing technique.<\/jats:p><\/jats:sec>Results<\/jats:title>We identified 24 patients with spindle cell melanoma, including 10 patients with desmoplastic melanoma, whose tumors had been analyzed for at least one of the three genes. The median Breslow thickness was 2.6 mm, and the most common site of the primary melanoma was the trunk, followed by the head and neck region. BRAF<\/jats:styled-content><\/jats:italic>, NRAS<\/jats:styled-content><\/jats:italic> and KIT<\/jats:styled-content><\/jats:italic> genomic sequencing was performed successfully in 20, 18 and 14 patients, respectively. Among the 20 melanomas with completed BRAF<\/jats:styled-content><\/jats:italic>\u2010sequencing analysis, 6 (30%) harbored a mutation, of which 5 (83%) had a V600E<\/jats:italic><\/jats:styled-content> mutation and 1 (17%) had a V600R<\/jats:italic><\/jats:styled-content> mutation. None of the melanomas harbored NRAS<\/jats:styled-content><\/jats:italic> or KIT<\/jats:styled-content><\/jats:italic> mutations.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>As has been reported in other common types of melanoma, V600<\/jats:italic><\/jats:styled-content> BRAF<\/jats:styled-content><\/jats:italic> mutation is the most common mutation of those tested in spindle cell melanoma. NRAS<\/jats:styled-content><\/jats:italic> or KIT<\/jats:styled-content><\/jats:italic> mutation appears to be rare, if not completely absent.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0560.2012.01950.x","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T12:26:31Z","timestamp":1342700791000},"page":"821-825","source":"Crossref","is-referenced-by-count":24,"title":["BRAF<\/scp><\/i>, NRAS<\/scp><\/i> and KIT<\/scp><\/i> sequencing analysis of spindle cell melanoma"],"prefix":"10.1111","volume":"39","author":[{"given":"Jinhyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander J.","family":"Lazar","sequence":"additional","affiliation":[{"name":"Department of Pathology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jade","family":"Homsi","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas E.","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010jen","family":"Hwu","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agop Y.","family":"Bedikian","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott E.","family":"Woodman","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sapna P.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Hwu","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin B.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.12-1-20"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20121"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(05)70019-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1987.tb05843.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1982.03330150042020"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1992.tb00957.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-199802000-00018"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00766"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.06.2984"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050092"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700026"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2004.10.032"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10185.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.746"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.14.0707"},{"issue":"8503","key":"e_1_2_6_17_1","first-page":"15s","article-title":"Phase I\/II study of GSK2118436, a selective inhibitor of oncogenic mutant BRAF kinase, in patients with metastatic melanoma and other solid tumors","volume":"28","author":"Kefford R","year":"2010","journal-title":"J Clin Oncol"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1103782"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1112302"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1002011"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20861"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-198905000-00003"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2005.04.038"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2010.090188"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0560.2012.01950.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0560.2012.01950.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T15:48:48Z","timestamp":1700581728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0560.2012.01950.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,19]]},"references-count":23,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1111\/j.1600-0560.2012.01950.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0560.2012.01950.x","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,19]]}}}