{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T06:48:31Z","timestamp":1699512511712},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T00:00:00Z","timestamp":1240531200000},"content-version":"vor","delay-in-days":11377,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scandinavian Journal of Haematology"],"published-print":{"date-parts":[[1978,3]]},"abstract":"<jats:p>Stroma from normal, AET\u2010treated and PNH red cells and their KCl\u2010extracts (partially purified on Sephadex G\u2010200) are able to trigger the activation of the alternative complement pathway. This fact has been demonstrated by:<\/jats:p><jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>\u2014 the lysis of PNH cells incubated in serum treated with stroma from normal or PNH\u2010RBC or with their extracts; the addition of Mg<jats:sup>2+<\/jats:sup> or Ca<jats:sup>2+<\/jats:sup> or of their chela\u2010tors (EDTA, EGTA) to the extract\u2010treated serum enhances or abolishes the lysis<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>\u2014 the reduction of complement activity in fresh serum incubated for 60' with PNH\u2010 extract<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>\u2014 the appearance of C<jats:sub>3<\/jats:sub> breakdown products in serum incubated with PNH\u2010extract, demonstrated by crossed immunoelectrophoresis.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p>In contrast, the same stroma (or extract) inhibits the sucrose lysis test, in which the lysis takes place through the classical complement pathway. No differences on the complement activation were observed between PNH and normal RBC stroma and between their chromatographic extracts.<\/jats:p><jats:p>These findings may suggest the possible role of diurnal variation of Mg<jats:sup>2+<\/jats:sup> and Ca<jats:sup>2+<\/jats:sup> concentration in precipitating haemolytic attacks and the possibility that small amount of circulating red cell stroma might maintain the haemolysis of PNH RBC.<\/jats:p>","DOI":"10.1111\/j.1600-0609.1978.tb02456.x","type":"journal-article","created":{"date-parts":[[2010,8,4]],"date-time":"2010-08-04T20:21:10Z","timestamp":1280953270000},"page":"265-270","source":"Crossref","is-referenced-by-count":0,"title":["Activation of Complement by Stroma from Normal and Paroxysmal Nocturnal Haemoglobinuria Red Cells"],"prefix":"10.1111","volume":"20","author":[{"given":"G.","family":"De Sandre","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Corrocher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. C.","family":"Falezza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Aprili","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Caffi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1975.tb01817.x"},{"key":"e_1_2_1_3_1","volume-title":"Practical haema\u2010tology","author":"Dacie J V","year":"1968"},{"key":"e_1_2_1_4_1","first-page":"293","article-title":"L'acetilcolinesterasi eritrocitaria","volume":"16","author":"Sandre G","year":"1956","journal-title":"Acta Med Patav"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.109.4.807"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197201272860403"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V35.4.462.462"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(69)90297-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"946","DOI":"10.4049\/jimmunol.111.3.946","article-title":"Extraction of complement inhibitory factors from the erythrocytes of non\u2010human species","volume":"111","author":"Hoffman E M","year":"1973","journal-title":"J Immunol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(65)90278-2"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107279"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the foliphenol reagent","volume":"193","author":"Lowry O H","year":"1951","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197310042891401"},{"key":"e_1_2_1_14_1","first-page":"334","article-title":"Influence of temperature and method of centrifugation on the separation of erythrocytes","volume":"82","author":"Murphy J R","year":"1973","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.138.3.715"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105389"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/215747a0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1182\/blood.V25.4.502.502","article-title":"The action of two sulphydryl compounds on normal human red cells. Relationship to red cells of paroxysmal nocturnal hemoglobinuria","volume":"25","author":"Sirchia G","year":"1965","journal-title":"Blood"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1159\/000458373","article-title":"Statistical evaluation of the determination of red cell acethylcholinesterase activity","volume":"11","author":"Sirchia G","year":"1970","journal-title":"En-zymol Biol Clin"}],"container-title":["Scandinavian Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0609.1978.tb02456.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0609.1978.tb02456.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T13:12:24Z","timestamp":1699449144000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0609.1978.tb02456.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,3]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1978,3]]}},"alternative-id":["10.1111\/j.1600-0609.1978.tb02456.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0609.1978.tb02456.x","archive":["Portico"],"relation":{},"ISSN":["0036-553X"],"issn-type":[{"value":"0036-553X","type":"print"}],"subject":[],"published":{"date-parts":[[1978,3]]}}}