{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T16:27:07Z","timestamp":1699720027596},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T00:00:00Z","timestamp":1240531200000},"content-version":"vor","delay-in-days":11316,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scandinavian Journal of Haematology"],"published-print":{"date-parts":[[1978,5]]},"abstract":"<jats:p>Samples of peripheral blood from patients with \u03b2\u2010thalassaemia major which contained significant numbers of nucleated normoblasts were stained with acridine orange and analyzed with rapid\u2010flow cytofluorometry. The pyknotic normoblast\u2010nuclei gave less green \u2018DNA\u2019 fluorescence than the (diploid) leucocytes and constituted a separate, distinct subpopulation. Mean values of the fluorescence intensities and standard deviations as displayed by multichannel analyses gave a numerical value for normoblasts with regard to their maturation stages. These mean values correlated with the differential counts of \u2018early and late\u2019 normoblasts in the light microscope under rigidly standardized conditions. Rapid\u2010flow cytofluorometry thus provides an objective and quantitative way to monitor and define peripheral blood normoblast populations as a measure of the severity of \u2018erythropoietic stress\u2019.<\/jats:p>","DOI":"10.1111\/j.1600-0609.1978.tb02480.x","type":"journal-article","created":{"date-parts":[[2010,8,4]],"date-time":"2010-08-04T16:12:31Z","timestamp":1280938351000},"page":"440-446","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of the Normoblast Population in \u03b2\u2010Thalassaemic Blood by Rapid\u2010Flow Cytofluorometry"],"prefix":"10.1111","volume":"20","author":[{"given":"Eliezer A.","family":"Rachmilewitz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Thorell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,24]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"289","article-title":"Machine characterization of human leukocytes by acridine orange fluorescence","volume":"15","author":"Adams L R","year":"1971","journal-title":"Acta Cytol"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.1932.01950030137017"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb04046.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1975.tb00870.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1182\/blood.V25.1.105.105","article-title":"Absorption spectra of inclusion bodies in beta\u2010thalassemia","volume":"25","author":"Fessas P","year":"1965","journal-title":"Blood"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.146.3643.530"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1974.tb00464.x"},{"key":"e_1_2_1_9_1","first-page":"441","article-title":"Denaturation of the normal and abnormal hemoglobin molecule","volume":"11","author":"Rachmilewitz E A","year":"1974","journal-title":"Semin in Hematol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1182\/blood.V47.3.495.495","article-title":"Lipid membrane peroxidation in beta thalassemia major","volume":"47","author":"Rachmilewitz E A","year":"1976","journal-title":"Blood"},{"issue":"267","key":"e_1_2_1_11_1","article-title":"Microfluorometric characteristics of intracellular nucleic acids and nucleoproteins by acridine orange","volume":"67","author":"Rigler R","year":"1966","journal-title":"Acta Phys Scand"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(79)90526-3"}],"container-title":["Scandinavian Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0609.1978.tb02480.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0609.1978.tb02480.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T12:25:36Z","timestamp":1699446336000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0609.1978.tb02480.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,5]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1978,5]]}},"alternative-id":["10.1111\/j.1600-0609.1978.tb02480.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0609.1978.tb02480.x","archive":["Portico"],"relation":{},"ISSN":["0036-553X"],"issn-type":[{"value":"0036-553X","type":"print"}],"subject":[],"published":{"date-parts":[[1978,5]]}}}