{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:42:39Z","timestamp":1791078159488,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T00:00:00Z","timestamp":1240531200000},"content-version":"vor","delay-in-days":5867,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> Neutrophil granule subsets and dynamics were studied in 4 patients with polycythemia vera\/myelofibrosis and 2 patients with chronic myelogenous leukemia. Alkaline phosphatase, a marker for the membrane of secretory vesicles (the most readily mobilizable pool of intracellular membranes in neutrophils) was highly elevated in the PV\/MF patients and significantly reduced in the CML patients. In spite of this, the amount of secretory vesicles was normal as judged by the content of albumin, and of the membrane protein cytochrome b<jats:sub>_245<\/jats:sub> and CD11b, both partially localized in secretory vesicles. Gelatinase granules were present in all patients. The azurophil granules were lighter than normal in both CML patients. SDS\u2010PAGE protein profiles indicated absence of defensins from azurophil granules from 1 CML patient. In addition, a 41\u201342 kD doublet protein band was absent from 2 PV and 1 CML patient, and reduced in the other CML patient. No difference in mobilization of granules was observed between patient neutrophils and control neutrophils. Also, stimulation with 10 <jats:sup>8<\/jats:sup> mol\/l <jats:italic>N<\/jats:italic>\u2010formyl\u2010methionyl\u2010leucyl\u2010phenylalanine induced normal increases in intracellular Ca<jats:sup>2+<\/jats:sup> in patient neutrophils. These results indicate that stimulus\u2010response coupling leading to granule exocytosis is intact in neutrophils from patients with myeloproliferative disorders.<\/jats:p>","DOI":"10.1111\/j.1600-0609.1993.tb01920.x","type":"journal-article","created":{"date-parts":[[2010,7,18]],"date-time":"2010-07-18T18:42:04Z","timestamp":1279478524000},"page":"189-199","source":"Crossref","is-referenced-by-count":13,"title":["Mobilization of granules in neutrophils from patients with myeloproliferative disorders"],"prefix":"10.1111","volume":"50","author":[{"given":"Niels","family":"Borregaard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Kjeldsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Sengel\u00f8v","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,4,24]]},"reference":[{"key":"e_1_2_2_2_2","first-page":"281A","article-title":"Hydroxyurea in the management of polycythemia vera: Analysis of long\u2010term leukemogenic potential","author":"Fruchtman SM","year":"1992","journal-title":"Clinical Research"},{"key":"e_1_2_2_3_2","first-page":"167","article-title":"Polycythemia Vera: An update II: Long\u2010term management of polycythemia vera with hydroxyurea: A progress report","volume":"23","author":"Kaplan ME","year":"1986","journal-title":"Semin Hematol"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1990.tb00344.x"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.97.1.52"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114509"},{"key":"e_1_2_2_7_2","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1182\/blood.V62.4.792.792","article-title":"Human neutrophils contain an intracellular pool of putative receptors for the chemoattractant N\u2010formyl\u2010methionyl\u2010leucyl\u2010phenylalanine","volume":"62","author":"Fletcher MP","year":"1983","journal-title":"Blood"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(82)90246-X"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.6.1641"},{"key":"e_1_2_2_10_2","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.4049\/jimmunol.135.2.1342","article-title":"Calcium requirements for increased complement receptor expression during neutrophil activation","volume":"135","author":"Berger M.","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113102"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2870603"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3629236"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114453"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42127-8"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1989.tb01254.x"},{"key":"e_1_2_2_17_2","unstructured":"CalafatJ. 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