{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:23:06Z","timestamp":1773300186746,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T00:00:00Z","timestamp":1240531200000},"content-version":"vor","delay-in-days":4802,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> The expression of molecules of the \u03b22 integrin family (CD11a, CD11b, CD11c and CD18) was explored on 2 human megakaryocytic cell lines and on platelets from different donors by immunofluorescence and flow cytometry using a large panel of mAb. CD11a, CD11b, CD11c and CD18 were detected on the megakaryocytic cell lines DAMI and HEL. A low and variable expression of CD11a, CD11b and CD18 determinants was also detected on resting platelets: this expression was markedly increased when platelets were activated by thrombin. Expression of CD18 was closely correlated to that of CD11a or CD11b when comparing the fluorescence intensity observed in different experiments. In presence of Ca<jats:sup>++<\/jats:sup>, platelets did bind to a RAJI cell line which exhibits a high expression level of CD54. This binding was increased when platelets were activated by thrombin and was decreased by an anti CD11a, CD18 and anti CD54 mAb. This study indicates that human platelets express molecules of the \u03b22 integrin family, when activated, which allows them to bind to CD54 bearing cells.<\/jats:p>","DOI":"10.1111\/j.1600-0609.1996.tb01331.x","type":"journal-article","created":{"date-parts":[[2010,7,18]],"date-time":"2010-07-18T13:01:33Z","timestamp":1279458093000},"page":"130-137","source":"Crossref","is-referenced-by-count":28,"title":["Activated human platelets express \u03b22 integrin"],"prefix":"10.1111","volume":"56","author":[{"given":"Marie Marthe","family":"Philippeaux","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Vesin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabienne","family":"Tacchini\u2010Cottier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre F.","family":"Piguet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1182\/blood.V80.6.1386.1386","article-title":"The molecular immunology of human platelet protein","volume":"80","author":"Kunicki TJ","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199303043280907"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60653-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/346425a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1182\/blood.V67.6.1757.1757","article-title":"Expression of the leukocyte functional molecule (LFA\u20101) on mouse platelets","volume":"67","author":"McCaffery PJ","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116470"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/147.2.435"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"4182","DOI":"10.1128\/iai.61.10.4182-4187.1993","article-title":"An effector role of platelets in the systemic and local LPS\u2010induced toxicity in mice, mediated by a CD11a\/CD54 dependent interaction with the endothelium","volume":"61","author":"Piguet PF","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_1_10_2","first-page":"321","article-title":"Pulmonary platelet trapping induced by bleomycin: correlation with fibrosis and involvement of the beta 2 integrins","volume":"75","author":"Piguet PF","year":"1994","journal-title":"Int J Exp Pathol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1182\/blood.V72.6.1968.1968","article-title":"Characterization of a new megakaryocytic cell line: the Dami cell","volume":"72","author":"Greenberg SM","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1002\/j.1460-2075.1984.tb01827.x","article-title":"Expression of platelet membrane glycoprotein and \u03b1\u2010granule proteins by a human erythroleukemia cell line (HEL)","volume":"3","author":"Tabilio A","year":"1984","journal-title":"EMBO J"},{"key":"e_1_2_1_13_2","first-page":"564","article-title":"Recommended method for indium\u2010111 platelet survival studies","volume":"29","author":"ICSH panel.","year":"1988","journal-title":"J Nucl Med"},{"key":"e_1_2_1_14_2","first-page":"14","article-title":"Shape and functional properties of human platelets washed with acid citrate","volume":"10","author":"Patscheke H","year":"1981","journal-title":"Haemo"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1985.tb02873.x"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1989.tb06275.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.2.423.423"},{"issue":"34","key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"18205","DOI":"10.1016\/S0021-9258(19)81346-7","article-title":"Complement proteins C5\u2010b9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity","volume":"263","author":"Sims PJ","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","unstructured":"AdelmanB CarlsonP HandinRI.Evaluation of platelet surface antigens by fluorescence flow cytometry. 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