{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T22:40:32Z","timestamp":1700260832871},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T00:00:00Z","timestamp":1240531200000},"content-version":"vor","delay-in-days":4649,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[1996,8]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> It has been reported that <jats:italic>in vitro<\/jats:italic> prednisolone (PDN) resistance provides a prognostic value in childhood acute lymphoblastic leukaemia (ALL). This study aimed at investigating the biological and clinical significance of <jats:italic>in vitro<\/jats:italic> PDN resistance in adult ALL. Blast cells from 30 patients were exposed to PDN (0.1 \u03bc<jats:sc>m<\/jats:sc>\u201335 \u03bc<jats:sc>m<\/jats:sc>) and cytotoxicity was determined by the soluble tetrazolium formazan 2,3\u2010bis (2\u2010methoxy\u20104\u2010nitro\u20105\u2010sulphophenyl)\u20105\u2010[(phenylamino) carbamyl]\u20102H\u2010tetrazolium hydroxyde (XTT) colorimetric assay. The IC<jats:sub>50<\/jats:sub> (defined as the drug concentration that results in 50% growth inhibition) varied greatly among the samples, from 0.3 \u03bc<jats:sc>m<\/jats:sc> to &gt;35 \u03bc<jats:sc>m<\/jats:sc>; 15 \u03bc<jats:sc>m<\/jats:sc> was subsequently chosen as IC<jats:sub>50<\/jats:sub>\u2010cut\u2010off point between <jats:italic>in vitro<\/jats:italic> resistant and sensitive cases. PDN\u2010induced cytotoxicity was significantly related to apoptosis, as demonstrated by regression analysis; in sensitive cases, however, the percentage of apoptotic cells after <jats:italic>in vitro<\/jats:italic> PDN treatment was significantly increased compared with control (<jats:italic>p<\/jats:italic>=0.002). Immunofluorescence evaluation of intracellular BCL\u20102 protein showed an equal percentage of positive cells in the two groups, but in resistant cells a higher mean fluorescence intensity (<jats:italic>p<\/jats:italic>=0.04) was demonstrated. <jats:italic>In vitro<\/jats:italic> sensitive and resistant patients did not display differences in clinical characteristics, in cytological, karyotypic and immunophenotypic features and in the outcome of induction therapy. Disease\u2010free survival (DFS), however, was significantly better in sensitive patients (<jats:italic>p<\/jats:italic>=0.02).<\/jats:p>","DOI":"10.1111\/j.1600-0609.1996.tb01351.x","type":"journal-article","created":{"date-parts":[[2010,7,18]],"date-time":"2010-07-18T13:47:23Z","timestamp":1279460843000},"page":"134-141","source":"Crossref","is-referenced-by-count":5,"title":["Biological and clinical significance of <i>in vitro<\/i> prednisolone resistance in adult acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"57","author":[{"given":"P.","family":"Tosi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Visani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Ottaviani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Manfroi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Tura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1182\/blood.V73.1.57.57","article-title":"Treatment of acute lymphoblastic leukaemia in adults with intensive induction, consolidation, and maintenance chemotherapy","volume":"73","author":"Hussein KK","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1200\/JCO.1990.8.6.994","article-title":"Results of vincristine, doxorubicin, and dexamethasone regimen in adults with standard\u2010 and high\u2010risk acute lymphocytic leukaemia","volume":"8","author":"Kantarjian HM","year":"1990","journal-title":"J Clin Oncol"},{"issue":"4","key":"e_1_2_1_4_2","first-page":"109","article-title":"Treatment of acute lymphoblastic leukemia: ALL\u201021 and ALL\u20103 EORTC studies","volume":"76","author":"Stryckmans P","year":"1991","journal-title":"Haematologica"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)91029-T"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-71213-5_21"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.132.1.38"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711580207"},{"key":"e_1_2_1_9_2","first-page":"491","article-title":"Involvement of BCL2 in glucocorticoid\u2010induced apoptosis of human pre\u2010B leukemias","volume":"52","author":"Alnemri ES","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/348334a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1182\/blood.V80.4.879.879","article-title":"BCL2 initiates a new category of oncogenes: regulators of cell death","volume":"80","author":"Korsmeyer SJ.","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_12_2","first-page":"225","article-title":"Expression of the BCL2 oncogene protein is not specific for the 14; 18 chromosomal translocation","volume":"137","author":"Pezzella F","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)93216-C"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1182\/blood.V81.11.3091.3091","article-title":"High expression of BCL2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy","volume":"81","author":"Campos L","year":"1993","journal-title":"Blood"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1994.tb04911.x"},{"issue":"2","key":"e_1_2_1_16_2","first-page":"182","article-title":"GIMEMA ALL 0288: a multicentric study on adult acute lymphoblastic leukemia. 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