{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:28:49Z","timestamp":1780471729183,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Abstract:<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> The <jats:italic>FLT3<\/jats:italic> gene is frequently mutated in acute myeloid leukemia (AML), either by an internal tandem duplication (ITD) of the juxtamembrane domain or by activating point mutations in the second tyrosine kinase domain (ATKD). Only a few investigations have focused on the prognostic significance of <jats:italic>FLT3<\/jats:italic> alterations in AML among the elderly, yielding conflicting results. In the present study, the frequency and clinical relevance of <jats:italic>FLT3<\/jats:italic> abnormalities were ascertained in a cohort of elderly AML patients.<\/jats:p><jats:p><jats:bold>Patients and methods:\u2002<\/jats:bold> A total of 109 AMLs, occurring in patients above the age of 60\u2003yr (median 71.5), were investigated. DNA was extracted from fresh bone marrow cells or from cells in fixative and investigated for the presence of ITD of exons 14 and 15 and the ATKD D835 in exon 20.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> ITDs and ATKDs were identified in 20 (18%) and 11 (10%) of the cases, respectively. Three cases displayed both an ITD and an ATKD. <jats:italic>FLT3<\/jats:italic> abnormalities were associated with leukocytosis (ITD <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01; ATKD <jats:italic>P<\/jats:italic>\u2003=\u20030.069), and the monocytic FAB subtypes M4 and M5 [ITD (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05), ATKD (<jats:italic>P<\/jats:italic>\u2003=\u20030.05)], and ITD and ATKD were significantly (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) more common in cases with a normal karyotype. There was no correlation between the presence of <jats:italic>FLT3<\/jats:italic> abnormalities and complete remission rates or overall survival.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> A correlation was observed between <jats:italic>FLT3<\/jats:italic> abnormalities and leukocytosis, a normal karyotype, and the M4\/M5 subtypes of leukemia. However, no clear\u2010cut prognostic impact of <jats:italic>FLT3<\/jats:italic> abnormalities was identified in elderly AML patients.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2004.00225.x","type":"journal-article","created":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T15:43:58Z","timestamp":1080834238000},"page":"307-313","source":"Crossref","is-referenced-by-count":44,"title":["Clinical impact of internal tandem duplications and activating point mutations in <i>FLT3<\/i> in acute myeloid leukemia in elderly 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