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We found that telomere fluorescence in MDS granulocytes, and CD34+ cells did not decline with age as in normal controls and that MDS granulocytes and CD34+ cells had significantly shorter telomeres than healthy controls. A significant higher incidence of cases with intermediate\u2010unfavourable cytogenetics and International Prognostic Scoring System (IPSS) int\u20102\/high\u2010risk group was observed among patients with lower telomere fluorescence. We also found that apoptosis in CD34+ cells was significantly higher in IPSS int\u20101 low\u2010risk patients when compared with IPSS int\u20102 high\u2010risk cases and healthy controls and that CD34+ cell telomere fluorescence directly correlated with CD34+ cell apoptosis. Reduced telomere fluorescence was associated with a history of occupational exposure to toxic agents and with worse survival in univariate and multivariate analyses. Our results suggest that flow\u2010cytometry assessment of telomere dynamics may represent a valuable tool in the biological and clinical\u2013prognostic characterisation of MDS disorders.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2004.00305.x","type":"journal-article","created":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T16:19:27Z","timestamp":1096561167000},"page":"351-358","source":"Crossref","is-referenced-by-count":28,"title":["Flow cytometric detection of accelerated telomere shortening in myelodysplastic syndromes: correlations with aetiological and clinical\u2013biological findings*"],"prefix":"10.1111","volume":"73","author":[{"given":"Gian Matteo","family":"Rigolin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo Della","family":"Porta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Bugli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Castagnari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endri","family":"Mauro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia Zenone","family":"Bragotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ciccone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Cuneo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluigi","family":"Castoldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,30]]},"reference":[{"key":"e_1_2_19_2_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1365-2141.1982.tb08475.x","article-title":"Proposals for the classification of myelodysplastic syndromes","volume":"51","author":"Bennett JM","year":"1982","journal-title":"Br J Haematol"},{"key":"e_1_2_19_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199905273402107"},{"key":"e_1_2_19_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-04-1199"},{"key":"e_1_2_19_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.12.3932"},{"key":"e_1_2_19_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.3.791"},{"key":"e_1_2_19_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402704"},{"key":"e_1_2_19_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14369-3"},{"key":"e_1_2_19_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/346866a0"},{"key":"e_1_2_19_10_2","first-page":"610","article-title":"Telomerase activity and telomere length in acute and chronic leukemia, pre\u2010 and post\u2010ex vivo culture","volume":"60","author":"Engelhardt M","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_19_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20761"},{"key":"e_1_2_19_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.979"},{"key":"e_1_2_19_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-01-0230"},{"key":"e_1_2_19_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2768"},{"key":"e_1_2_19_15_2","first-page":"402","article-title":"Immunophenotypic analysis of myelodysplastic syndromes","volume":"88","author":"Del Canizo MC","year":"2003","journal-title":"Haematologica"},{"key":"e_1_2_19_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0898-743"},{"key":"e_1_2_19_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.2.157"},{"key":"e_1_2_19_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.6.1883"},{"key":"e_1_2_19_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.4.895"},{"key":"e_1_2_19_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990200413"},{"key":"e_1_2_19_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402293"},{"key":"e_1_2_19_22_2","volume-title":"ISCN. 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