{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T11:53:46Z","timestamp":1700654026860},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,8,5]],"date-time":"2005-08-05T00:00:00Z","timestamp":1123200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Partial tandem (PTD) and internal tandem duplications (ITD) of the <jats:italic>MLL<\/jats:italic> or <jats:italic>FLT3<\/jats:italic> genes respectively, have been demonstrated in acute myeloid leukemia (AML). While occurrence of each of these PTD\/ITD seem to confer an unfavorable prognosis, the literature contains only sparse information of the occurrence and the prognosis of simultaneous PTD\/ITD of these genes. We have therefore attempted to determine the presence and its consequence in AML and with the further aim of characterizing such patients with respect to other genetic aberrations and to prototype variables in this disease. We analyzed blast cells from 250 adult patients treated at the same institution during a 15\u2010year period for <jats:italic>FLT3<\/jats:italic> ITD and <jats:italic>MLL<\/jats:italic> PTD and the duplications were found in 24% and 4%, respectively. The four co\u2010duplicated cases (2%) did not differ with respect to sex, age, FAB\u2010type, or immunophenotype, promoter methylation of <jats:italic>p<\/jats:italic>15, <jats:italic>E\u2010cadherin<\/jats:italic> (<jats:italic>CDH1<\/jats:italic>), <jats:italic>Estrogen receptor<\/jats:italic>, <jats:italic>MDR1<\/jats:italic>, expression of apoptosis\u2010related or multidrug resistance\u2010related genes, though a trend toward decreased gene expression of MDR1 was observed. Two of the patients had a normal karyotypic analysis, while the remaining two showed aberrations in chromosome 11, one with trisomy 11 and the other with a der (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#b11\">11<\/jats:ext-link>). The extensive molecular characterization of <jats:italic>FLT3\/MLL<\/jats:italic> coduplicated patients presented here indicates that, even though they do not differ molecularly from the groups of patients with single ITDs, their prognosis and overall survival is universally poor. More patients are needed to determine whether coduplication has independent clinical implications compared to patients with single ITD\/PTD.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2005.00502.x","type":"journal-article","created":{"date-parts":[[2005,8,5]],"date-time":"2005-08-05T16:26:59Z","timestamp":1123259219000},"page":"185-192","source":"Crossref","is-referenced-by-count":12,"title":["Delineation and molecular characterization of acute myeloid leukemia patients with coduplication of <i>FLT3<\/i> and <i>MLL<\/i>"],"prefix":"10.1111","volume":"75","author":[{"given":"Lene Hyldahl","family":"Olesen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte Guldborg","family":"Nyvold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anni","family":"Aggerholm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan 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