{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:20:47Z","timestamp":1770218447030,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T00:00:00Z","timestamp":1132272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold>  <jats:italic>Objectives:<\/jats:italic>\u2002To make humanized monoclonal antibodies by phage surface display technology, we screened out the specific anti\u2010platelet glycoproteins (GPs) IgG antibody from patients with chronic idiopathic thrombocytopenic purpura (ITP), which can inhibit platelet aggregation. <jats:italic>Methods:<\/jats:italic>\u2002We studied plasmas from 68 patients with ITP for the presence of IgG antibodies specific for GPIIb\/IIIa and\/or GPIb\/IX using modified monoclonal antibody immobilization of platelet antigen assays. The IgG antibody and its F(ab\u2032)<jats:sub>2<\/jats:sub> fragments of the positive plasmas which could inhibit platelet aggregation function were prepared and purified. Their immunoreactivity to platelet GPs and effects on platelet function were further analyzed.<jats:italic>Results:<\/jats:italic>\u2002GPIIb\/IIIa\u2010 and GPIb\/IX\u2010specific antibodies were found in 21 and 19 patients, respectively. Six of them had antibodies against both GP complexes. Among the 34 positive plasmas, four with positive anti\u2010GPIIb\/IIIa autoantibody showed significant inhibition of platelet aggregation induced by adenosine diphosphate (ADP), whereas one with GPIb\/IX\u2010specific antibody inhibited ristocetin\u2010induced platelet aggregation. The purified IgG and its F(ab\u2032)<jats:sub>2<\/jats:sub> fragments from two patients not only retained the ability to bind to platelet GPs but also impaired the <jats:italic>in vitro<\/jats:italic> ADP\u2010induced platelet aggregation.<jats:italic>Conclusions:<\/jats:italic>\u2002F(ab\u2032)<jats:sub>2<\/jats:sub> portion of the IgG is a functional fragment, which is responsible for the autoantibody interaction with platelet GPs in ITP, and some of them also affect platelet function, which can be used to develop completely humanized anti\u2010GPIIb\/IIIa small molecular phage antibody.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2005.00557.x","type":"journal-article","created":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T10:36:45Z","timestamp":1132310205000},"page":"153-159","source":"Crossref","is-referenced-by-count":12,"title":["Effects of IgG and its F(ab\u2032)<sub>2<\/sub> fragments of some patients with idiopathic thrombocytopenic purpura on platelet 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