{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:03:59Z","timestamp":1761894239505},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,2,6]],"date-time":"2006-02-06T00:00:00Z","timestamp":1139184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold>  <jats:italic>Objectives<\/jats:italic>:\u2002Diffuse large B\u2010cell lymphoma (DLBCL) is the most common form of non\u2010Hodgkin lymphomas. Cytogenetic studies have revealed a broad spectrum of clonal genetic abnormalities and complex karyotypes. The purpose of this study was to contribute to the understanding of the genomic alterations associated with this group of lymphomas. <jats:italic>Methods<\/jats:italic>:\u2002Cytogenetic, fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) and molecular analyses were performed in 30 cases with DLBCL: 20 <jats:italic>de novo<\/jats:italic> DLBCL (<jats:italic>dn<\/jats:italic>\u2010DLBCL) and 10 DLBCL secondary to follicular lymphoma (S\u2010DLBCL). <jats:italic>Results<\/jats:italic>:\u2002A total of 37 different structural chromosomal rearrangements were found: 27% translocations, 54% deletions, and 19% other alterations. Chromosomes 8, 6, 2, and 9 were the most commonly affected. Interestingly, translocation t(3;14)(q27;q32) and\/or <jats:italic>BCL\u20106<\/jats:italic> gene rearrangements were not observed either by cytogenetic studies or by FISH analysis. Fifteen novel cytogenetic alterations were detected, among them translocations t(2;21)(p11;q22) and t(8;18)(q24;p11.3) appeared as sole structural abnormalities. Translocation t(14;18)(q32;q21) and\/or <jats:italic>BCL\u20102\u2010IGH<\/jats:italic> gene rearrangements were the genomic alterations most frequently observed: 50% of S\u2010DLBCL and 30% of <jats:italic>dn<\/jats:italic>\u2010DLBCL. Deletions del(4)(q21), del(6)(q27), del(8)(q11), and del(9)(q11) were recurrent. The most common gains involved chromosome regions at 12q13\u2010q24, 7q10\u2010q32, and 17q22\u2010qter; 6q was the most frequently deleted region, followed by losses at 2q35\u2010qter, 7q32\u2010qter, and 9q13\u2010qter. Four novel regions of loss were identified: 5q13\u2010q21, 2q35\u2010qter (both recurrent in our series), 4p11\u2010p12, and 17q11\u2010q12. <jats:italic>Conclusions<\/jats:italic>:\u2002These studies emphasize the value of combining conventional cytogenetics with FISH and molecular studies to allow a more accurate definition of the genomic aberrations involved in DLBCL.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2005.00616.x","type":"journal-article","created":{"date-parts":[[2006,2,6]],"date-time":"2006-02-06T15:34:36Z","timestamp":1139240076000},"page":"284-293","source":"Crossref","is-referenced-by-count":9,"title":["New chromosome abnormalities and lack of <i>BCL\u20106<\/i> gene rearrangements in Argentinean diffuse large B\u2010cell lymphomas"],"prefix":"10.1111","volume":"76","author":[{"given":"Roxana","family":"Cerretini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria F.","family":"Noriega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Narbaitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irma","family":"Slavutsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,6]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"118","volume-title":"Pathology & Genetics Tumours of Haematopietic and Lymphoid Tissues. 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