{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:12:10Z","timestamp":1792509130041,"version":"4.5.0"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T00:00:00Z","timestamp":1139961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold>  <jats:italic>Objectives<\/jats:italic>:\u2002Investigate the contribution and mechanism of cell\u2010mediated cytotoxicity to the pathogenesis of idiopathic thrombocytopenic purpura (ITP). <jats:italic>Methods<\/jats:italic>:\u2002We observed the cytotoxic effect of cytotoxic T\u2010lymphocyte (CTL) (CD8<jats:sup>+<\/jats:sup>) and natural killer cells (CD3<jats:sup>\u2212<\/jats:sup>CD16<jats:sup>+<\/jats:sup>CD56<jats:sup>+<\/jats:sup>) toward chronic ITP patient's autologous platelets, and investigated the expression of Fas ligand (FasL), tumor necrosis factor (TNF)\u2010<jats:italic>\u03b1<\/jats:italic> and TNF\u2010related apoptosis inducing ligand, as well as perforin and granzyme B mRNA in CD8<jats:sup>+<\/jats:sup> cells using flow cytometry and reverse transcriptase\u2010polymerase chain reaction. <jats:italic>Results<\/jats:italic>:\u2002We found that platelet lysis was seen only using purified CD8<jats:sup>+<\/jats:sup> T cells as effector cells; expression of FasL and TNF\u2010<jats:italic>\u03b1<\/jats:italic> in CD8<jats:sup>+<\/jats:sup> T cells in ITP group was elevated. Moreover, the mRNA levels of granzyme B and perforin in CD8<jats:sup>+<\/jats:sup> cells of ITP patients were increased. <jats:italic>Conclusions<\/jats:italic>:\u2002Our findings suggest that CTLs are activated in chronic ITP and might be involved in the pathogenesis of this disorder. Apoptosis and perforin\/granzyme\u2010mediated cytotoxicity constitute an important pathway through which CTLs destruct autologous platelets. CTLs might be a reasonable target for a therapeutic strategy.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2005.00622.x","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T11:28:55Z","timestamp":1140002935000},"page":"427-431","source":"Crossref","is-referenced-by-count":143,"title":["Cell\u2010mediated lysis of autologous platelets in chronic idiopathic thrombocytopenic purpura"],"prefix":"10.1111","volume":"76","author":[{"given":"Feng","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoxin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra010501"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90111-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-08-2672"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1182\/blood.V81.5.1246.1246","article-title":"Autoantibodies against platelet glycoproteins in autoimmune thrombocytopenic purpura: their clinical significance and response to treatment","volume":"81","author":"Berchtold P","year":"1993","journal-title":"Blood"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm921"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00038-6"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90102-1"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-07-2268"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1182\/blood.V100.5.1774.h81702001774_1774_1779","article-title":"Expression patterns of Th1 and Th2 cytokine genes in childhood idiopathic thrombocytopenic purpura (ITP) at presentation and their modulation by intravenous immunoglobulin G (IVIg) treatment: their role in prognosis","volume":"100","author":"Mouzaki A","year":"2002","journal-title":"Blood"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-0502(03)00041-7"},{"key":"e_1_2_6_12_2","first-page":"615","article-title":"Role of Fas\u2010FasL in insulitis in nonobese diabetic mouse","volume":"117","author":"Cao JY","year":"2004","journal-title":"Chin Med J (Engl)"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2001.5017"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00756.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.5.855"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1160\/TH04-06-0385","article-title":"Disturbed apoptosis of T\u2010cells in patients with active idiopathic thrombocytopenic purpura","volume":"93","author":"Olsson B","year":"2005","journal-title":"Thromb Haemost"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0609.2005.00622.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0609.2005.00622.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T04:23:37Z","timestamp":1696998217000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0609.2005.00622.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,15]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1600-0609.2005.00622.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0609.2005.00622.x","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,15]]}}}