{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:13:18Z","timestamp":1758820398943},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Interleukin\u20105 (IL\u20105) promotes signal transduction and expansion of eosinophil colonies in bone marrow via interactions with its heterodimeric receptor (IL\u20105R). Two variants encoding soluble forms of the alpha subunit (sIL\u20105R<jats:italic>\u03b1<\/jats:italic>) have been described, although the signals promoting and\/or limiting differential transcription remain to be clarified. <jats:italic>Objectives<\/jats:italic>:\u2002Our intent was to explore the role of IL\u20105 in regulating differential transcription of these splice variants <jats:italic>in vivo<\/jats:italic>. <jats:italic>Methods<\/jats:italic>:\u2002We have designed a quantitative reverse transcriptase\u2010polymerase chain reaction assay to detect transcripts encoding the transmembrane, soluble 1 and 2 forms of IL\u20105R<jats:italic>\u03b1<\/jats:italic> in two strains of wild\u2010type (BALB\/c and C57BL\/6) and corresponding IL\u20105 gene\u2010deleted mice. Wild\u2010type mice respond to <jats:italic>S. mansoni<\/jats:italic> infection with a gradual increase in serum IL\u20105 and eosinophilia, which is not observed in IL\u20105 gene\u2010deleted mice. <jats:italic>Results and conclusions<\/jats:italic>:\u2002We find that IL\u20105 is not necessary for differential splicing to occur <jats:italic>in vivo<\/jats:italic>, as all three forms of the IL\u20105R<jats:italic>\u03b1<\/jats:italic> are detected in both strains of IL\u20105 gene\u2010deleted mice, with ratios of transcript expression (transmembrane\u2003:\u2003soluble 1\u2003:\u2003soluble 2) that were indistinguishable from their wild\u2010type counterparts. Differential splicing does vary markedly between strains, potentially because of local effects of strain\u2010specific polymorphisms.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2006.00699.x","type":"journal-article","created":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T15:13:05Z","timestamp":1153408385000},"page":"181-190","source":"Crossref","is-referenced-by-count":6,"title":["Interleukin\u20105 does not influence differential transcription of transmembrane and soluble isoforms of IL\u20105R<i>\u03b1 in vivo<\/i>"],"prefix":"10.1111","volume":"77","author":[{"given":"Jonas","family":"Bystr\u00f6m","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly D.","family":"Dyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suk See 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