{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T16:23:12Z","timestamp":1697473392122},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold>  <jats:italic>Objectives:<\/jats:italic>\u2002Burkitt's lymphoma (BL) is a highly aggressive mature B\u2010cell neoplasm comprising endemic, sporadic and immunodeficiency\u2010associated variants. Human cell lines constitute a very useful tool to investigate the biology of lymphoid neoplasia. In this study, we succeeded in establishing two human cell lines, GAL\u201001 and GAL\u201002, from a HIV\u2010negative patient with Epstein\u2010Barr virus (EBV) \u2010negative sporadic BL presenting as an effusion. GAL\u201001 and GAL\u201002 were established at diagnosis and after one course of polychemotherapy, respectively. The <jats:italic>in vivo<\/jats:italic> effusion occurred in a very peculiar clinical setting; the patient having a previous history of intestinal diffuse large B\u2010cell lymphoma. <jats:italic>Methods:<\/jats:italic>\u2002The morphologic, immunophenotypic and molecular genetic features of GAL cell lines are reported and compared with those of the parental tumour. The findings clearly demonstrated that the Burkitt effusion did not represent disease progression of the intestinal tumour, but represented a second primary haematological malignancy. The <jats:italic>in vivo<\/jats:italic> tumorigenic properties of the cells were tested by subcutaneous injection to NOD\/SCID mice. <jats:italic>Results:<\/jats:italic>\u2002Both cell lines were composed of medium\u2010sized lymphoid cells with clumped chromatin, multiple medium\u2010sized nucleoli and moderate amounts of vacuolated cytoplasm. GAL cells display the phenotype and genotype of a B\u2010cell lineage (positive for CD20, CD79a and clonal rearrangement of Ig heavy chain), carry the c\u2010MYC rearrangement by t(8;22)(q24;q11) translocation and are characterised by the expression of the germinal centre\u2010associated antigens CD10, BCL6, CD38 and absent to low BCL2 expression. EBV and HHV8 were not identified within parental tumour or in cultured cells. Subcutaneous injection of both cell lines to NOD\/SCID mice induced tumour formation. <jats:italic>Conclusions:<\/jats:italic>\u2002GAL\u201001 and GAL\u201002, two novel EBV\u2010negative human BL cell lines represent a potentially useful experimental model to study the biology of BL possibly including the resistance to chemotherapy.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2006.00724.x","type":"journal-article","created":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T15:13:05Z","timestamp":1153408385000},"page":"318-326","source":"Crossref","is-referenced-by-count":1,"title":["Establishment and characterisation of two novel human KSHV\u2010 and EBV\u2010negative Burkitt cell lines, GAL\u201001 and GAL\u201002, from a primary lymphomatous effusion"],"prefix":"10.1111","volume":"77","author":[{"given":"Caroline","family":"Thielen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Herens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010France","family":"Fassotte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Detrooz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Drion","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Diss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Boniver","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"De Leval","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,19]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60470-4"},{"key":"e_1_2_7_3_2","volume-title":"Pathology and genetics of tumours of haematopoietic and lymphoid tissues","author":"Jaffe ES","year":"2001"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204595"},{"key":"e_1_2_7_5_2","volume-title":"The leukemia\u2010lymphoma cell line. 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