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For this, we employed the two\u2010phase liquid culture procedure for growing erythroid progenitors, high performance liquid chromatography for analysis of HbF production and reverse transcription polymerase chain reaction for quantification of the accumulation of globin mRNAs. The results demonstrated that rapamycin induced an increase of HbF in cultures from all the <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia patients studied and an increase of their overall Hb content\/cell. The inducing effect of rapamycin was restricted to <jats:italic>\u03b3<\/jats:italic>\u2010globin mRNA accumulation, being only minor for <jats:italic>\u03b2<\/jats:italic>\u2010globin and none for <jats:italic>\u03b1<\/jats:italic>\u2010globin mRNAs. The ability of rapamycin to preferentially increase <jats:italic>\u03b3<\/jats:italic>\u2010globin mRNA content and production of HbF in erythroid precursor cells from <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia patients is of great importance as this agent (also known as sirolimus or rapamune) is already in clinical use as an anti\u2010rejection agent following kidney transplantation. These data suggest that rapamycin warrants further evaluation as a potential therapeutic drug in <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia and sickle cell anaemia.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2006.00731.x","type":"journal-article","created":{"date-parts":[[2006,8,29]],"date-time":"2006-08-29T16:58:53Z","timestamp":1156870733000},"page":"437-441","source":"Crossref","is-referenced-by-count":82,"title":["Effects of rapamycin on accumulation of <i>\u03b1<\/i>\u2010, <i>\u03b2<\/i>\u2010 and <i>\u03b3<\/i>\u2010globin mRNAs in erythroid precursor cells from <i>\u03b2<\/i>\u2010thalassaemia 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