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A rapid technique for diagnosis of \u03b1\u2010thalassemia\u20101 SEA type was implemented. The technique used is based on real\u2010time gap\u2010PCR and high resolution melting (HRM) analysis of the amplified fragment using the Rotor\u2010Gene 6000\u2122. The DNA samples used for amplification were obtained from whole blood, cord blood, and chorionic villus sampling (CVS). With this method, the \u03b1\u2010thalassemia\u20101 SEA allele can be easily distinguished from wild type \u03b1\u2010globin gene allele. The real\u2010time gap\u2010PCR and HRM analysis offers additional benefits including minimal labor, rapid turnaround time, and a decreased risk of PCR carryover contamination. It is cost\u2010effective and safe, does not require fluorescently labeled probe and hazardous chemicals. Moreover, it is accurate showing 100% concordance with conventional gap\u2010PCR analysis.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2008.01055.x","type":"journal-article","created":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T15:20:49Z","timestamp":1202916049000},"page":"510-514","source":"Crossref","is-referenced-by-count":95,"title":["Detection of alpha\u2010thalassemia\u20101 Southeast Asian type using real\u2010time gap\u2010PCR with SYBR Green1 and high resolution melting 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