{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:42:54Z","timestamp":1790678574989,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,11,5]],"date-time":"2008-11-05T00:00:00Z","timestamp":1225843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The death\u2010associated protein kinase 1 (<jats:italic>DAPK1<\/jats:italic>) gene is a candidate tumor suppressor (TSG) and the abnormal methylation of <jats:italic>DAPK1<\/jats:italic> gene has been found in many carcinomas. The epigenetic changes of TSGs are now recognized as a mechanism contributing to the development of chronic myeloid leukemia (CML). To clarify the role of <jats:italic>DAPK1<\/jats:italic> in CML, we examined the methylation status of <jats:italic>DAPK1<\/jats:italic> in 49 patients with CML using methylation\u2010specific polymerase chain reaction. The aberrant methylation of the <jats:italic>DAPK1<\/jats:italic> gene was found in 25 of 49 (51.0%) CML cases, not in all controls. No correlation was found between <jats:italic>DAPK1<\/jats:italic> gene methylation and the age, hematologic parameters, chromosomal abnormalities, the types and levels of bcr\/abl transcripts of CML patients. However, correlation could be observed between the sex and the status of <jats:italic>DAPK1<\/jats:italic> methylation in CML patients (<jats:italic>R<\/jats:italic>\u2003=\u20030.374, <jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.008). Furthermore, there was a significant correlation between <jats:italic>DAPK1<\/jats:italic> methylation and the stages of CML (<jats:italic>R<\/jats:italic>\u2003=\u20030.354, <jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.013). The CML patients in accelerated phase (AP) and blast crisis (BC) had higher frequency of <jats:italic>DAPK1<\/jats:italic> methylation than those in chronic phase (CP) (75.0% vs. 34.5%) (\u03c7<jats:sup>2<\/jats:sup>\u2003=\u20037.776, <jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.005). In one patient, the status of <jats:italic>DAPK1<\/jats:italic> methylation became positive on the transition from CP to AP and BC. These results suggested that <jats:italic>DAPK1<\/jats:italic> promoter methylation might play a significant role in the progression of CML.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2008.01178.x","type":"journal-article","created":{"date-parts":[[2008,11,6]],"date-time":"2008-11-06T03:38:28Z","timestamp":1225942708000},"page":"119-123","source":"Crossref","is-referenced-by-count":41,"title":["Aberrant methylation of the death\u2010associated protein kinase 1 (<i>DAPK1<\/i>) CpG island in chronic myeloid leukemia"],"prefix":"10.1111","volume":"82","author":[{"given":"Jun","family":"Qian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010ming","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010rong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao\u2010yang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.4065\/81.7.973"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2147"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-12-4111"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06604.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0919"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11543"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22470"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.103004.142615"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.1.15"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.12.4347"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.043"},{"key":"e_1_2_6_13_2","first-page":"1724","article-title":"Frequent death\u2010associated protein kinase promoter hypermethylation in multiple myeloma","volume":"7","author":"Ng MH","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-07-2249"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207505"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0609.2008.01178.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0609.2008.01178.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T19:53:31Z","timestamp":1696967611000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0609.2008.01178.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,8]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1111\/j.1600-0609.2008.01178.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0609.2008.01178.x","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,8]]}}}