{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T13:36:57Z","timestamp":1715953017971},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,28]],"date-time":"2009-01-28T00:00:00Z","timestamp":1233100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Plasma has been found to be enriched with tumor\u2010specific DNA, RNA, and protein in patients with hematologic disease. We assessed the utility of plasma as a DNA source for detection of genetic abnormalities in patients with suspected B\u2010 or T\u2010cell lymphoproliferative disorders.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> DNA was extracted from paired peripheral blood (PB) cells and plasma for polymerase chain reaction (PCR)\u2010based detection of immunoglobulin heavy chain (<jats:italic>IgH<\/jats:italic>) and T\u2010cell receptor gamma chain (<jats:italic>TCR\u2010\u03b3<\/jats:italic>) rearrangements, and B\u2010cell leukemia\/lymphoma (<jats:italic>BCL<\/jats:italic>)\u2010<jats:italic>1\/IgH<\/jats:italic> and <jats:italic>BCL\u20102<\/jats:italic>\/<jats:italic>IgH<\/jats:italic> translocations.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Concordance between plasma and PB cell analysis was 100% for <jats:italic>IgH<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2003=\u200357), <jats:italic>TCR\u2010\u03b3<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2003=\u200357), and <jats:italic>BCL\u20101<\/jats:italic>\/<jats:italic>IgH<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2003=\u200337) rearrangements, and 94% (60\/64) for <jats:italic>BCL\u20102<\/jats:italic>\/<jats:italic>IgH<\/jats:italic>; four of 11 plasma samples positive for <jats:italic>BCL\u20102<\/jats:italic>\/<jats:italic>IgH<\/jats:italic> tested negative in paired cells. No plasma or PB cell samples from 195 healthy donors showed genetic abnormalities.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> These findings indicate that plasma is a reliable sample type for detection of abnormalities associated with B\u2010 and T\u2010cell lymphoproliferative disorders, providing sensitivity equal to or greater than that of PB cells.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2009.01231.x","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T03:06:08Z","timestamp":1233198368000},"page":"450-453","source":"Crossref","is-referenced-by-count":4,"title":["Plasma\u2010based detection of clonality in lymphoid malignancies"],"prefix":"10.1111","volume":"82","author":[{"given":"Chen\u2010Hsiung","family":"Yeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maher","family":"Albitar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,21]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1373\/49.4.562"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1373\/49.8.1286"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.055434"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0996-1033"},{"key":"e_1_2_4_6_2","first-page":"1659","article-title":"DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells","volume":"61","author":"Jahr S","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(01)00665-9"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2002.169"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402016"},{"key":"e_1_2_4_10_2","first-page":"3251","article-title":"Presence of tumor DNA in plasma of breast cancer patients: clinicopathological correlations","volume":"59","author":"Silva JM","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.22.1805"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0996-1035"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402743"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0609.2003.00125.x"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.12.4953"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1994.tb04828.x"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-06-1840"},{"key":"e_1_2_4_18_2","first-page":"297","article-title":"Molecular aspects of chromosomal translocation (14;18)","volume":"30","author":"Lee MS","year":"1993","journal-title":"Semin Hematol"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.1.315"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(02)00091-8"},{"key":"e_1_2_4_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.1148"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0609.2009.01231.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0609.2009.01231.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T19:45:29Z","timestamp":1696967129000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0609.2009.01231.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,21]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1111\/j.1600-0609.2009.01231.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0609.2009.01231.x","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,21]]}}}