{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:25:15Z","timestamp":1791033915269,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T00:00:00Z","timestamp":1250812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Vaso\u2010occlusive crisis (VOC) is a significant cause of morbidity and mortality in sickle cell anemia (SCA) patients. Insofar as polymorphism in human platelet alloantigen (HPA) exhibit a prothrombotic nature, we hypothesized that specific HPA polymorphic variants are associated with VOC. We investigated the distribution of HPA1, HPA2, HPA3, HPA4, and HPA5 alleles genotypes among VOC and non\u2010VOC control SCA patients.<\/jats:p><jats:p><jats:bold>Patients\/methods:\u2002<\/jats:bold> This was a case\u2013control study. Study subjects comprised SCA patients with (VOC group; <jats:italic>n<\/jats:italic>\u2003=\u2003127) or without (Steady\u2010state group; <jats:italic>n<\/jats:italic>\u2003=\u2003130) VOC events. HPA genotyping was done by PCR\u2010SSP.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Significantly higher frequencies of HPA\u20102b, HPA\u20103b, and HPA\u20105b alleles, and marked enrichment of HPA\u20103b\/3b, HPA\u20105a\/5b, and HPA\u20105b\/5b genotypes, were seen in VOC than in control SCA patients. Taking homozygous wild\u2010type genotypes as reference, univariate analysis identified HPA\u20103a\/3b, HPA\u20103b\/3b, and HPA\u20105b\/5b to be associated with VOC. Multivariate analysis confirmed the independent association of only HPA\u20103a\/3b and HPA\u20103b\/3b genotypes with VOC. HPA\u20103 genotypes were significantly correlated with VOC frequency, type, and medication, and requirement for hospitalization. While both HPA 3a\/3b (<jats:italic>P\u2003<\/jats:italic>=\u20030.002; OR\u2003=\u20032.94; 95% CI\u2003=\u20031.49\u20135.77) and 3b\/3b (<jats:italic>P\u2003<\/jats:italic>=\u20030.006; OR\u2003=\u20033.16; 95% CI\u2003=\u20031.40\u20137.17) genotypes were associated with need for hospitalization, only HPA\u20103b\/3b was associated with VOC frequency, type (localized vs. generalized), and medication (narcotics vs. NSAIDs).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> This confirms the association of HPA polymorphisms with SCA VOC, of which HPA\u20103 appears to be independent genetic risk factors for SCA VOC.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2009.01339.x","type":"journal-article","created":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T15:21:00Z","timestamp":1250868060000},"page":"579-585","source":"Crossref","is-referenced-by-count":8,"title":["Human platelet alloantigens (HPA) 1, HPA2, HPA3, HPA4, and HPA5 polymorphisms in sickle cell anemia patients with vaso\u2010occlusive crisis"],"prefix":"10.1111","volume":"83","author":[{"given":"Abeer M.","family":"Al\u2010Subaie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naglaa A.","family":"Fawaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najat","family":"Mahdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman K.","family":"Al\u2010Absi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadija","family":"Al\u2010Ola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada","family":"Ameen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wassim 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