{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T15:50:54Z","timestamp":1714751454064},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,11,6]],"date-time":"2009-11-06T00:00:00Z","timestamp":1257465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Minimal residual disease (MRD) detection during the early treatment phase has become an important stratification parameter in many childhood acute lymphoblastic leukaemia (ALL) treatment protocols. Here, we aimed to address the applicability of rearranged antigen\u2010receptor genes as potential MRD markers using real\u2010time quantitative polymerase chain reaction (RQ\u2010PCR) in a Swedish population\u2010based cohort. From 334 childhood ALL cases diagnosed during 2002\u20132006, we analysed 279 diagnostic samples (84%) by screening for rearranged immunoglobulin (IG) and T\u2010cell receptor (TCR) genes. Allele\u2010specific oligonucleotides were designed, and the sensitivity and quantitative level was determined for each target. Overall, clonal IG\/TCR rearrangements were detected in 97% (236\/244) of B\u2010cell precursor ALL (BCP ALL) and 94% (33\/35) of T\u2010ALL. A sensitive RQ\u2010PCR analysis (\u226410<jats:sup>\u22124<\/jats:sup>) was obtained in 89% (216\/244) of BCP ALL and in 74% (26\/35) of T\u2010ALL, whereas two sensitive targets were only available in 47% (115\/244) of BCP ALL and 29% (10\/35) of T\u2010ALL cases. With the stratification threshold of \u226510<jats:sup>\u22123<\/jats:sup>, which is applied in the current Nordic treatment protocol (NOPHO\u2010ALL 2008) for the identification of high\u2010risk patients, 93% of BCP ALL and 86% of T\u2010ALL reached this quantitative range by at least one target gene. Taken together, this national retrospective study demonstrates that an IG\/TCR target for MRD monitoring can be identified in the majority of childhood ALL cases, whereas identification of a second sensitive target gene needs to be improved.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2009.01374.x","type":"journal-article","created":{"date-parts":[[2009,11,6]],"date-time":"2009-11-06T11:46:34Z","timestamp":1257507994000},"page":"117-127","source":"Crossref","is-referenced-by-count":9,"title":["Applicability of IG\/TCR gene rearrangements as targets for minimal residual disease assessment in a population\u2010based cohort of Swedish childhood acute lymphoblastic leukaemia diagnosed 2002\u20132006"],"prefix":"10.1111","volume":"84","author":[{"given":"Ingrid","family":"Th\u00f6rn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Forestier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Britt","family":"Thuresson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carina","family":"Wasslavik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Malec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elenor","family":"Lindstr\u00f6m\u2010Eriksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Botling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisela","family":"Barbany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Jacobsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tor","family":"Olofsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Porwit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christer","family":"Sundstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Rosenquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)04058-6"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808273390904"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.3.790.003k48_790_794"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1253"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.6.1952"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402548"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403038"},{"key":"e_1_2_6_9_2","first-page":"319","article-title":"Rearrangements of IgH, TCRD and TCRG genes as clonality marker of childhood acute lymphoblastic leukemia","volume":"27","author":"Meleshko AN","year":"2005","journal-title":"Exp Oncol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.5"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401277"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403505"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1182\/blood.V74.1.334.334","article-title":"Rearrangement and expression of T\u2010cell receptor delta genes in T\u2010cell acute lymphoblastic leukemias","volume":"74","author":"Van Dongen JJ","year":"1989","journal-title":"Blood"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401765"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403263"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1999.tb01765.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401970"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403008"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1455"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403444"},{"key":"e_1_2_6_21_2","first-page":"1783","article-title":"Proposals for the immunological classification of acute leukemias. 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