{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T01:10:36Z","timestamp":1758935436382},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T00:00:00Z","timestamp":1269302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p> <jats:italic>Objectives:<\/jats:italic>\u2002APAF\u20101 is a central component of the intrinsic pathway of apoptosis, where APAF\u20101 dysregulation results in the development of diverse human neoplasms. The aim of this study was to characterize the mRNA expression levels of <jats:italic>APAF\u20101<\/jats:italic> transcripts in low\u2010risk and high\u2010risk MDS and to elucidate whether the expression levels of <jats:italic>APAF\u20101<\/jats:italic> transcripts are modulated with increased apoptosis in CD34<jats:sup>+<\/jats:sup> MDS cells undergoing erythroid differentiation. <jats:italic>Methods: APAF\u20101<\/jats:italic> (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"NM_181861\">NM_181861<\/jats:ext-link>) expression was verified, by quantitative RT\u2010PCR, in bone marrow aspirates from 33 patients with myelodysplastic syndromes (MDS), at the time of diagnosis, and in erythroid differentiation cultures from CD34<jats:sup>+<\/jats:sup> from normal donors and patients with MDS. <jats:italic>Results: APAF\u20101<\/jats:italic> expression was significantly higher in low\u2010risk, compared to high\u2010risk MDS, according to IPSS (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.0001), FAB (<jats:italic>P<\/jats:italic>\u2003=\u20030.0265), and cytogenetic risk (<jats:italic>P<\/jats:italic>\u2003=\u20030.0134). Low\u2010risk MDS\u2010derived differentiated erythroid cells demonstrated an increased expression of <jats:italic>APAF\u20101<\/jats:italic>, compared with normal cells, accompanied by an augmented rate of apoptosis. <jats:italic>Conclusions:<\/jats:italic> Increased expression of <jats:italic>APAF\u20101<\/jats:italic> in low\u2010risk disease and its positive correlation with the apoptotic rate observed during the erythroblast differentiation of low\u2010risk MDS cells may indicate that the modulation of <jats:italic>APAF\u20101<\/jats:italic>, at the transcriptional level, participates in the pathophysiology of MDS.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2010.01429.x","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T10:06:33Z","timestamp":1269338793000},"page":"525-530","source":"Crossref","is-referenced-by-count":4,"title":["Increased expression of <i>APAF\u20101<\/i> in low\u2010risk myelodysplastic syndrome: a possible role in the pathophysiology of myelodysplasia"],"prefix":"10.1111","volume":"84","author":[{"given":"Bruno Deltreggia","family":"Benites","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola","family":"Traina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana da Silva Santos","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene Gyongyver H.","family":"Lorand\u2010Metze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Ferreira","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara Teresinha","family":"Saad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,5,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2047"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02994003"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428190009054877"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(98)00112-X"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-06-1774"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02581.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:APPT.0000045786.98031.1d"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.2.414"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21535"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1594"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/35051606"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2005.01.044"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208022"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.11.4275.4275"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.1.268.bloodjournal861268"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1640"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.09.030"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2008000700004"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200310041"}],"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0609.2010.01429.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0609.2010.01429.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T09:57:31Z","timestamp":1698746251000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0609.2010.01429.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,17]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1111\/j.1600-0609.2010.01429.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0609.2010.01429.x","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,17]]}}}