{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,14]],"date-time":"2024-04-14T15:05:54Z","timestamp":1713107154067},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,3,23]],"date-time":"2011-03-23T00:00:00Z","timestamp":1300838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective:\u2002<\/jats:bold> Chronic lymphocytic leukemia (CLL) is incurable, but therapy leading to eradication of minimal residual disease (MRD) in CLL is associated with improved clinical outcomes. CLL upregulated gene 1 (<jats:italic>CLLU1<\/jats:italic>) is solely upregulated in CLL patient samples. We hypothesized that <jats:italic>CLLU1<\/jats:italic> could be used to monitor for residual disease in CLL patient samples after therapy.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We examined whether the <jats:italic>CLLU1<\/jats:italic> real\u2010time quantitative PCR (RQ\u2010PCR) could detect small numbers of CLL cells in mixtures of normal peripheral blood mononuclear (PBMC) cells. We then performed a retrospective analysis on time\u2010matched cryo\u2010preserved specimens from patients who achieved MRD\u2010negative remissions that underwent serial marrow biopsies for evaluation of residual disease by 4\u2010color flow cytometry. RNA from PBMC samples collected at the time of the marrow assessments was analyzed for <jats:italic>CLLU1<\/jats:italic>. Nine patients underwent a total of 46 paired blood and marrow evaluations (median 5 assessments per patient).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>  <jats:italic>CLLU1<\/jats:italic> RQ\u2010PCR on PBMCs of healthy donors reconstituted with varying amounts of CLL cells demonstrated leukemia cells could be reliably detected with high sensitivities depending on the <jats:italic>CLLU1<\/jats:italic> expression level. Analysis of time\u2010matched samples assessed for <jats:italic>CLLU1<\/jats:italic> levels in the blood by RQ\u2010PCR and residual disease of the marrow determined by 4\u2010color flow cytometry revealed a correlation coefficient of 0.96 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.0001).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The <jats:italic>CLLU1<\/jats:italic> RQ\u2010PCR is a sensitive and specific assay for detecting residual CLL cells after therapy. Assessment of blood <jats:italic>CLLU1<\/jats:italic> levels can be used as a reliable marker of tumor burden and has the potential to complement currently used techniques for MRD monitoring in patients with CLL.<\/jats:p>","DOI":"10.1111\/j.1600-0609.2011.01588.x","type":"journal-article","created":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T22:08:20Z","timestamp":1297807700000},"page":"405-411","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of <i>CLLU1<\/i> expression levels before and after therapy in patients with chronic lymphocytic leukemia"],"prefix":"10.1111","volume":"86","author":[{"given":"Anne Mette","family":"Buhl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danelle 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