{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T12:24:00Z","timestamp":1759580640637},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T00:00:00Z","timestamp":1345507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Mantle cell lymphoma (<jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>) is one of the most heterogeneous lymphoid neoplasms with a variable course of disease. Although t(11;14)(q13;q32) is the hallmark of <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content> resulting in cyclin D1 (<jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic>) overexpression in 90% of patients, this is difficult to validate by immunohistochemistry. We hypothesised that <jats:italic><jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>11<\/jats:italic> could be a robust molecular biomarker for <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We have developed very sensitive and specific RT\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> assay employing a poly\u2010A specific RT primer to circumvent contamination from <jats:styled-content style=\"fixed-case\">gDNA<\/jats:styled-content> caused by the intron\u2010less nature of <jats:italic>SOX11<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found a significant difference between the expression levels of <jats:italic><jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>11<\/jats:italic> in patients with <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content> at diagnosis (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>21) and in healthy donors (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>18) (blood: <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.0001; marrow: <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.0001). <jats:italic><jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>11<\/jats:italic> expression of very low levels close to the assay sensitivity was detected in only 2 of 18 healthy donors, while low levels of <jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic> expression was observed in all blood and 12 of 13 marrow samples within the defined detection limit of Cq\u00a0=\u00a040. In spiking experiments of the <jats:styled-content style=\"fixed-case\">GRANTA<\/jats:styled-content>\u2010519 <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content> cell line into mononuclear cells from normal donor, the sensitivity of the <jats:italic><jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>11<\/jats:italic> assay was found to be 2\u00a0\u00d7\u00a010<jats:sup>\u22124<\/jats:sup>, while the sensitivity of the <jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic> assay was estimated to 2\u00a0\u00d7\u00a010<jats:sup>\u22123<\/jats:sup> because of the normal background expression. In longitudinal sampling from patients with <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content> the minimal residual disease (<jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content>) values based on the <jats:italic><jats:styled-content style=\"fixed-case\">SOX<\/jats:styled-content>11<\/jats:italic> expression mirrored the clinical disease development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This <jats:italic>SOX11<\/jats:italic> RT\u2010qPCR assay could be a useful tool for MRD monitoring in patients with MCL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0609.2012.01837.x","type":"journal-article","created":{"date-parts":[[2012,7,24]],"date-time":"2012-07-24T12:55:29Z","timestamp":1343134529000},"page":"385-394","source":"Crossref","is-referenced-by-count":18,"title":["A highly sensitive and specific <scp>qPCR<\/scp> assay for quantification of the biomarker <i>SOX11<\/i> in mantle cell 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