{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:07:20Z","timestamp":1758632840804},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,19]],"date-time":"2006-01-19T00:00:00Z","timestamp":1137628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold> Microchimerism of persistent fetal cells has been implicated in some cell\u2010mediated autoimmune diseases. This study examines the hypothesis that fetal microchimerism plays a role in the pathogenesis of lichen planus (LP) affecting the oral cavity.<\/jats:p><jats:p><jats:bold>Study design:\u2002<\/jats:bold> Mucosal biopsies of 12 women with oral LP (OLP) were tested for the presence of both male cells and male DNA originating from prior pregnancies or prior blood transfusions. Six male patients with OLP served as a control group. Biopsies were analyzed for the presence of Y\u2010chromosome\u2010positive cells by fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) with X\u2010 and Y\u2010specific DNA probes. To confirm the FISH findings, we used fluorescent polymerase chain reaction (PCR) to identify Y\u2010chromosome sequences in DNA extracted from mucosal lesions.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Using FISH technology, all the 18 biopsy samples showed good hybridization results. In females, Y\u2010chromosome\u2010specific signals were not detected by FISH at any site of the lesions. PCR amplification demonstrated a single peak at the locus specific for the X\u2010chromosome.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Male DNA microchimerism was not present in any of the investigated lesions, suggesting that microchimerism because of persisting male fetal cells is unlikely to play a major role in the pathogenesis of OLP.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2006.00393.x","type":"journal-article","created":{"date-parts":[[2006,1,19]],"date-time":"2006-01-19T18:43:31Z","timestamp":1137696211000},"page":"125-129","source":"Crossref","is-referenced-by-count":7,"title":["Role of microchimerism in the pathogenesis of oral lichen planus"],"prefix":"10.1111","volume":"15","author":[{"given":"W.","family":"Weger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Odell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Pertl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Cerroni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kerl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Jakse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Pertl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,19]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"463","volume-title":"Fitzpatrick's Dermatology in General Medicine","author":"Daoud M S","year":"2003"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090010501"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(00)00142-5"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8628(20000306)91:1<22::AID-AJMG4>3.0.CO;2-3"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199804233381704"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)08357-8"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08688-9"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000051560"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2001.300703.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2165\/00066982-200005030-00007"},{"key":"e_1_2_6_12_2","first-page":"288","volume-title":"Methods in Molecular Medicine. 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