{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:27:46Z","timestamp":1791080866261,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,6,15]],"date-time":"2007-06-15T00:00:00Z","timestamp":1181865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Dendritic cells (DCs) need to mobilize within the extracellular matrix (ECM) during their maturation and concomitant migration from peripheral sites to lymphoid organs. Syndecans are cell surface proteoglycans that mediate the interaction of DCs with the ECM. Here we investigated the influence of syndecans on dendritic cell motility and morphology. Langerhans cells of the epidermis and monocyte\u2010derived DCs were found to undergo a switch in syndecan expression during maturation. Syndecan\u20101 was downregulated and syndecan\u20104 was strongly upregulated within the first hours of lipopolysaccharide\u2010induced dendritic cell maturation and during Langerhans cell emigration from human skin, as shown by flow cytometry and qRT\u2010PCR. Syndecan\u20101 downregulation was inhibited by syndecan\u20104 siRNA knock\u2010down, indicating a functional interconnection between enhanced syndecan\u20104 expression and syndecan\u20101 downregulation. Syndecan\u20104 upregulation is functionally involved in dendritic cell motility, as inhibition of syndecan\u20104 function by means of blocking antibodies or through siRNA knock\u2010down decreased dendritic cell motility. In other experiments, the cytoskeletal component a\u2010actinin was observed to be upregulated in DCs as a consequence of the induction of maturation, and was found to colocalize with syndecan\u20104. Furthermore, lammellopodial spreading by DCs on fibronectin (FN)\u2010coated surfaces was dependent on syndecan\u20104. This binding of syndecan\u20104 to FN and its association with the cytoskeleton may be relevant for syndecan\u20104\u2010dependent dendritic cell motility. We conclude that the switch in syndecan expression during dendritic cell maturation controls the motility of DCs in a way that appears to be crucial for their mobilization from peripheral sites and subsequent migration to lymphoid tissues.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2007.00568.x","type":"journal-article","created":{"date-parts":[[2007,6,16]],"date-time":"2007-06-16T13:58:27Z","timestamp":1182002307000},"page":"580-589","source":"Crossref","is-referenced-by-count":23,"title":["Switch in syndecan\u20101 and syndecan\u20104 expression controls maturation associated dendritic cell motility"],"prefix":"10.1111","volume":"16","author":[{"given":"Marco","family":"Averbeck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Gebhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Anderegg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Termeer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan P.","family":"Sleeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan 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