{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T17:16:02Z","timestamp":1762017362077},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,3,6]],"date-time":"2009-03-06T00:00:00Z","timestamp":1236297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> The melanin\u2010concentrating hormone receptor 1 (MCHR1) has been identified as a B cell autoantigen in vitiligo with antibodies to the receptor detectable in binding and function\u2010blocking assays. Two epitope domains (amino acids 1\u2013138 and 139\u2013298) have been previously identified. In this study, we aimed to further define the epitope specificity of MCHR1 antibodies using phage\u2010display technology and to identify the epitopes recognised by receptor antibodies detected in MCHR1 function\u2010blocking assays. Antibody reactivity to MCHR1 peptides 51\u201380, 85\u201398, 154\u2013158 and 254\u2013260 was identified by phage\u2010display and subsequently confirmed in phage ELISA in 2\/12, 5\/12, 3\/12 and 6\/12 of vitiligo patients, respectively. The results suggest that major autoantibody epitopes are localised in the 85\u201398 and 254\u2013260 amino acid regions of MCHR1 with minor epitopes in amino acid sequences 51\u201380 and 154\u2013158. Antibodies with MCHR1 function\u2010blocking activity were determined to recognise epitope 254\u2013260, this being the first epitope to be reported as a target site for antibodies that block the function of the receptor.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2008.00813.x","type":"journal-article","created":{"date-parts":[[2009,3,6]],"date-time":"2009-03-06T07:24:22Z","timestamp":1236324262000},"page":"454-463","source":"Crossref","is-referenced-by-count":11,"title":["Mapping of melanin\u2010concentrating hormone receptor 1 B cell epitopes predicts two major binding sites for vitiligo patient autoantibodies"],"prefix":"10.1111","volume":"18","author":[{"given":"Nikos G.","family":"Gavalas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raju V. S. R. 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