{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:47:46Z","timestamp":1783572466196,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,8,5]],"date-time":"2010-08-05T00:00:00Z","timestamp":1280966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:p> <jats:italic>Please cite this paper as: In vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> analysis of pro\u2010 and anti\u2010inflammatory effects of weak and strong contact allergens. Experimental Dermatology 2010; <jats:bold>19<\/jats:bold>: 1007\u20131013.<\/jats:p><jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Inflammation is a crucial step in the development of allergic contact dermatitis. The primary contact with chemical allergens, called sensitization, and the secondary contact, called elicitation, result in an inflammatory response in the skin. The ability of contact allergens to induce allergic contact dermatitis correlates to a great extent with their inflammatory potential. Therefore, the analysis of the sensitizing potential of a putative contact allergen should include the examination of its ability and potency to cause an inflammation. In this study, we examined the inflammatory potential of different weak contact allergens and of the strong sensitizer 2,4,6\u2010trinitrochlorobenzene (TNCB) <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> using the contact hypersensitivity model, the mouse model for allergic contact dermatitis. Cytokine induction was analysed by PCR and ELISA to determine mRNA and protein levels, respectively. Inflammation\u2010dependent recruitment of skin\u2010homing effector T cells was measured in correlation with the other methods. We show that the sensitizing potential of a contact allergen correlates with the strength of the inflammatory response. The different methods used gave similar results. Quantitative cytokine profiling may be used to determine the sensitizing potential of chemicals for hazard identification and risk assessment.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2010.01136.x","type":"journal-article","created":{"date-parts":[[2010,8,5]],"date-time":"2010-08-05T19:11:38Z","timestamp":1281035498000},"page":"1007-1013","source":"Crossref","is-referenced-by-count":24,"title":["<i>In vitro<\/i> and <i>in vivo<\/i> analysis of pro\u2010 and anti\u2010inflammatory effects of weak and strong contact allergens"],"prefix":"10.1111","volume":"19","author":[{"given":"Christian","family":"Lass","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irmgard","family":"Merfort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan F.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,8,5]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000078765"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02082.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/138955706776073493"},{"key":"e_1_2_7_5_2","first-page":"173","article-title":"Innate and adaptive immune responses in contact dermatitis: analogy with infections","volume":"144","author":"Freudenberg M A","year":"2009","journal-title":"G Ital Dermatol Venereol"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00919.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.2.857"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425817"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2000.01126.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1999.8841"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2001.9286"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002040100267"},{"key":"e_1_2_7_13_2","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1006\/taap.2002.9473","article-title":"Cytokine production induced by low\u2010molecular\u2010weight chemicals as a function of the stimulation index in a modified local lymph node assay: an approach to discriminate contact sensitizers from respiratory sensitizers","volume":"184","author":"Van Och F M","year":"2002","journal-title":"Toxicol Appl Pharmacol"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.07.055"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/71.2.137"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070509"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.150.9.3698"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2000.9085"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00360.x"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021908"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.10.6390"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2006.02330.x"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3283088cf9"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3283079ea4"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1503"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.03.017"},{"key":"e_1_2_7_27_2","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.4049\/jimmunol.151.5.2390","article-title":"Inhibition of Langerhans cell antigen\u2010presenting function by IL\u201010. 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