{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:33:30Z","timestamp":1773423210099,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T00:00:00Z","timestamp":1328832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Pemphigus vulgaris (PV) is an autoimmune blistering skin disease characterized by suprabasal acantholysis and by autoantibodies against desmoglein 3 localized on desmosomes. In addition, caspases also seem to participate in this blistering disease. Focal adhesion kinase (FAK) is a non\u2010receptor tyrosine kinase involved in cytoskeleton remodelling and formation and disassembly of cell adhesion structures. We have previously demonstrated that HER (human epidermal growth factor receptor related) isoforms, Src (Rous sarcoma) and mammalian target of rapamycin (mTOR), three molecules implicated in signalling processes, take part in suprabasal acantholysis and apoptosis induced by PV\u2010IgG in a mouse model. Our aim was to investigate whether upregulation of FAK is implicated in the development of PV lesions. Herein, using a mouse model, PV\u2010IgG administration showed an increased level of FAK phosphorylated on 397 and 925 tyrosine residues in the basal layer of epidermis. When mice were pretreated with a FAK inhibitor (FI), the acantholysis of the basal layer of epidermis was absent. More interestingly, we observed that phosphorylated FAK (Y397\/925) decreased when HER isoforms, Src, mTOR and pan\u2010caspases inhibitors were employed before PV\u2010IgG administration. In addition, pretreatment with the FI before PV\u2010IgG injection prevented the changes in both Bax and Bcl\u20102 expression and caspase\u20109 and caspase\u20103 activities induced by PV\u2010IgG. Finally, FI reduced the expression of phosphorylated Src and mTOR in the basal cells of epidermis. In conclusion, our data reveal a novel role of phosphorylated FAK (Y397\/925) in PV development involving HER isoforms, Src and mTOR kinases.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2012.01441.x","type":"journal-article","created":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T18:06:54Z","timestamp":1326305214000},"page":"254-259","source":"Crossref","is-referenced-by-count":35,"title":["Inhibition of FAK prevents blister formation in the neonatal mouse model of pemphigus vulgaris"],"prefix":"10.1111","volume":"21","author":[{"given":"Mar\u00eda P.","family":"Gil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Modol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agust\u00edn","family":"Espa\u00f1a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda 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LanzaA PrimeS.Apoptosis\u2010independent caspase activation as an early pathogenic event in the autoimmune skin disease pemphigus vulgaris. Poster presented in JC Bystryn pemphigus & pemphigoid meeting: from the bench to the bedside. 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