{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T06:05:53Z","timestamp":1789452353991,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T00:00:00Z","timestamp":1334707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Our objective here was to perform a quantitative phosphoproteomic study on a reconstituted human skin tissue to identify low\u2010 and high\u2010dose ionizing radiation\u2010dependent signalling in a complex three\u2010dimensional setting. Application of an isobaric labelling strategy using sham and three radiation doses (3, 10, 200\u2003cGy) resulted in the identification of 1052 unique phosphopeptides. Statistical analyses identified 176 phosphopeptides showing significant changes in response to radiation and radiation dose. Proteins responsible for maintaining skin structural integrity including keratins and desmosomal proteins (desmoglein, desmoplakin, plakophilin 1, 2 and 3) had altered phosphorylation levels following exposure to both low and high doses of radiation. Altered phosphorylation of multiple sites in profilaggrin linker domains coincided with altered profilaggrin processing suggesting a role for linker phosphorylation in human profilaggrin regulation. These studies demonstrate that the reconstituted human skin system undergoes a coordinated response to both low and high doses of ionizing radiation involving multiple layers of the stratified epithelium that serve to maintain tissue integrity and mitigate effects of radiation exposure.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2012.01470.x","type":"journal-article","created":{"date-parts":[[2012,2,14]],"date-time":"2012-02-14T06:41:34Z","timestamp":1329201694000},"page":"352-357","source":"Crossref","is-referenced-by-count":21,"title":["Quantitative phosphoproteomics identifies filaggrin and other targets of ionizing radiation in a human skin model"],"prefix":"10.1111","volume":"21","author":[{"given":"Feng","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina 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