{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T21:01:42Z","timestamp":1781730102125,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T00:00:00Z","timestamp":1334707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Antibodies, specific to murine DEC205, can be used to target antigens to dendritic cells. The immunodominant domain of human type XVII collagen, hNC16A, was fused to this antibody (DEC\u2010hNC16A) and was administered as expression plasmid by gene gun transfection with the aim of inducing tolerance to human type XVII collagen in a skin transplantation model. Mice transfected with DEC\u2010hNC16A were challenged with skin grafts from transgenic mice engineered to express human type XVII collagen. Graft survival was either prolonged or grafts were accepted infinitely (33% and 16%, respectively) upon treatment with DEC\u2010hNC16A while 100% of grafts were rejected in untreated controls. Graft acceptance was associated with the absence of a CD4+ infiltrate and a dense CD8+ T\u2010cell infiltrate and was not strictly dependent on antibody production. Our results show that DEC\u2010hNC16A targets dendritic cells <jats:italic>in vivo<\/jats:italic> leading to prolonged survival of transgenic skin grafts. This indicates that DEC205\u2010targeting may be used for the induction of tolerance to skin antigens, which would increase the chances of successful skin gene therapy of epidermolysis bullosa patients.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2012.01474.x","type":"journal-article","created":{"date-parts":[[2012,2,17]],"date-time":"2012-02-17T18:06:51Z","timestamp":1329502011000},"page":"395-398","source":"Crossref","is-referenced-by-count":18,"title":["Targeting of the hNC16A collagen domain to dendritic cells induces tolerance to human type XVII collagen"],"prefix":"10.1111","volume":"21","author":[{"given":"Monika","family":"Ettinger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris K.","family":"Gratz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Gruber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Hauser\u2010Kronberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theron S.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Mahnke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Thalhamer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Hintner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Peckl\u2010Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann W.","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,18]]},"reference":[{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R400034200"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)00003-1"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2005.01937.x"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.2003.01192.x"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06988.x"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2009.00792.x"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004030050392"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12337492"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2010.01080.x"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2007.08.008"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021598"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.6.769"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.3.673"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3229"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700970"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198605000-00001"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011603"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.107"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0625.2012.01474.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0625.2012.01474.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T06:30:38Z","timestamp":1698647438000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0625.2012.01474.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,18]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1111\/j.1600-0625.2012.01474.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0625.2012.01474.x","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,18]]}}}