{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:25:42Z","timestamp":1791188742351,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,4,16]],"date-time":"2012-04-16T00:00:00Z","timestamp":1334534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Cyclooxygenase\u20102 (COX\u20102) is an enzyme induced in response to multiple mitogenic and inflammatory stimuli, including UV light. UV\u2010induced COX\u20102 expression induces production of prostaglandin E2 (PGE2) in keratinocytes, which mediates inflammation and cell proliferation. Until recently, studies regarding COX\u20102 and PGE2 in the skin have focused on keratinocytes and skin cancer and the effect of PGs produced by keratinocytes on melanocytes. However, the effects of COX\u20102 itself or COX\u20102 inhibitors on melanogenesis are not well known. Therefore, to establish the role of COX\u20102 in melanogenesis, we investigated the effects of knock\u2010down of COX\u20102 in melanocytes on melanin production and the expression of melanogenic molecules through silencing of COX\u20102 expression with COX\u20102 short interfering RNA (siRNA). COX\u20102 knock\u2010down in melanocytes decreased the expressions of tyrosinase, TRP\u20101, TRP\u20102, gp100 and MITF and also reduced tyrosinase enzyme activity. Furthermore, COX\u20102 siRNA\u2010transfected melanocytes showed markedly reduced alpha\u2010melanocyte stimulating hormone (\u03b1\u2010MSH)\u2010induced melanin production. In addition, \u03b1\u2010MSH\u2010induced COX\u20102 expression in both scrambled siRNA\u2010transfected and COX\u20102 siRNA\u2010transfected melanocytes was greater than \u03b1\u2010MSH\u2010untreated cells. Our results suggest that COX\u20102 might be a candidate target for the development of anti\u2010melanogenic agents and \u03b1\u2010MSH\u2010induced pigmentation could be closely associated with COX\u20102 expression. COX\u20102 inhibitors might therefore be of particular use in whitening cosmetics for hyperpigmentation disorders such as melasma, postinflammatory hyperpigmentation and solar lentigo.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2012.01483.x","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T02:07:51Z","timestamp":1330999671000},"page":"420-425","source":"Crossref","is-referenced-by-count":47,"title":["siRNA\u2010mediated knock\u2010down of COX\u20102 in melanocytes suppresses melanogenesis"],"prefix":"10.1111","volume":"21","author":[{"given":"Ji Y.","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Y.","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miri 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