{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:32:34Z","timestamp":1761708754669},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,7,10]],"date-time":"2012-07-10T00:00:00Z","timestamp":1341878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transient knock\u2010down of the gap junction protein <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 by antisense and si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, or gap junction block with mimetic peptides, have been shown to enhance epidermal wound healing. However, patients with oculodentodigital dysplasia (<jats:styled-content style=\"fixed-case\">ODDD<\/jats:styled-content>) express mutant <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 that leads to a chronic reduction in gap junctional intercellular communication. To determine whether mutant <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 in keratinocytes would impact upon the wound healing process, we localized <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 in human and mouse skin tissue expressing mutant <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 and assessed the ability of primary keratinocytes derived from a mouse model of <jats:styled-content style=\"fixed-case\">ODDD<\/jats:styled-content> to proliferate, migrate and differentiate. In the epidermis from an <jats:styled-content style=\"fixed-case\">ODDD<\/jats:styled-content> patient and in the epidermis of mice expressing the <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>60<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content> mutant or in keratinocytes obtained from mutant mice, <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 was frequently found within intracellular compartments and rarely localized to punctate sites of cell\u2013cell apposition. Primary keratinocytes derived from <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>60<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content> mutant mice proliferated faster but migrated similarly to keratinocytes derived from wild\u2010type control mice. Keratinocytes derived from mutant mice expressed abundant <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 and higher levels of involucrin and loricrin under low calcium conditions. However, after calcium\u2010induced differentiation, similar levels of <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43, involucrin and loricrin were observed. Thus, we conclude that during wound healing, mutant <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>x43 may enhance keratinocyte proliferation and promote early differentiation of keratinocytes.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2012.01532.x","type":"journal-article","created":{"date-parts":[[2012,5,11]],"date-time":"2012-05-11T16:45:56Z","timestamp":1336754756000},"page":"612-618","source":"Crossref","is-referenced-by-count":20,"title":["The <scp>G<\/scp>60<scp>S C<\/scp>x43 mutant enhances keratinocyte proliferation and differentiation"],"prefix":"10.1111","volume":"21","author":[{"given":"Jared M.","family":"Churko","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacinda","family":"Sampson","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Utah School of Medicine Salt Lake City UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donglin","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale W.","family":"Laird","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University of Western Ontario London ON Canada"},{"name":"Department of Physiology and Pharmacology University of Western Ontario London ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100155"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15419060500514143"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/346090"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20958"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6399"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22046"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn203"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002000102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22529.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.6.1735"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1091\/mbc.6.11.1491","volume":"6","author":"Goliger J A","year":"1995","journal-title":"Mol Biol Cell"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200404142"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1065-6995(03)00077-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03320"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01057.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.09.007"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21472"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02011"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12532899"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-380-0_22"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.056093"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.009514"},{"key":"e_1_2_7_24_1","first-page":"193","volume":"289","author":"Matic M","year":"2005","journal-title":"Methods Mol Biol"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700786"},{"issue":"1","key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"S23","DOI":"10.1089\/sur.2002.3.s1-23","volume":"3","author":"Hackam D J","year":"2002","journal-title":"Surg Infect (Larchmt)"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2010.08.004"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmq047"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.058750"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20404"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.080217"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600026200"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2009.11.001"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00471.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701125"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-08-0737"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.14.6453"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703623200"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.09.013"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.30412"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.31558"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.01013.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.09.004"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0625.2012.01532.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0625.2012.01532.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T21:24:29Z","timestamp":1698701069000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0625.2012.01532.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,10]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1111\/j.1600-0625.2012.01532.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0625.2012.01532.x","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,10]]}}}