{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:37:54Z","timestamp":1790318274416,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,8,17]],"date-time":"2012-08-17T00:00:00Z","timestamp":1345161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>S\u00e9zary syndrome (<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>) is an aggressive cutaneous <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell lymphoma with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+ tumor cells localized in the skin, lymph nodes and peripheral blood. Characteristic molecular aberrancies in <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> have been identified; however, paucity of functional models severely hampered the translation of these observations into pathogenic mechanisms, and subsequent validation of novel therapeutic targets. We therefore developed a mouse model for <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> using intrahepatic injection of <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> cells in newborn immunodeficient <jats:styled-content style=\"fixed-case\">RAG<\/jats:styled-content>2<jats:sup>\u2212\/\u2212<\/jats:sup> \u03b3c<jats:sup>\u2212\/\u2212<\/jats:sup> mice that are completely devoid of T\u2010, B\u2010 and <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>\u2010cell activity. Injection of the <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> cell line SeAx led to long\u2010term and reproducible systemic repopulation of the mice. Injection of mice with the <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> cell line HuT\u201078 led to the death of the mice owing to massive growth of internal tumors. Four weeks after injection of primary <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> cells, human <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3+ T cells could be tracked back in the liver, peripheral blood, lymph nodes, spleen and skin of the mice, although the engraftment rate varied when using cells from different patients. In conclusion, we demonstrate that injection of <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> cell lines or primary cells in newborn <jats:styled-content style=\"fixed-case\">RAG<\/jats:styled-content>2<jats:sup>\u2212\/\u2212<\/jats:sup> \u03b3c<jats:sup>\u2212\/\u2212<\/jats:sup> mice results in long\u2010term systemic repopulation of the mice, thereby providing a novel mouse model for <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u00e9zary syndrome.<\/jats:p>","DOI":"10.1111\/j.1600-0625.2012.01556.x","type":"journal-article","created":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T16:25:29Z","timestamp":1340382329000},"page":"706-709","source":"Crossref","is-referenced-by-count":22,"title":["A novel mouse model for <scp>S<\/scp>\u00e9zary <scp>S<\/scp>yndrome using xenotransplantation of <scp>S<\/scp>\u00e9zary cells into immunodeficient <scp>RAG<\/scp>2<sup>\u2212\/\u2212<\/sup> \u03b3c<sup>\u2212\/\u2212<\/sup> mice"],"prefix":"10.1111","volume":"21","author":[{"given":"Leslie","family":"van der Fits","sequence":"first","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heggert G.","family":"Rebel","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacoba J.","family":"Out\u2010Luiting","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan M.","family":"Pouw","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology Academic Medical Center of the University of Amsterdam Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Smit","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim G.","family":"Vermeer","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisanne","family":"van Zijl","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis P.","family":"Tensen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kees","family":"Weijer","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology Academic Medical Center of the University of Amsterdam Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten H.","family":"Vermeer","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2010.551159"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1182\/blood.V93.1.260","volume":"93","author":"Qin J 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