{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T08:47:48Z","timestamp":1698742068670},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,7,5]],"date-time":"2004-07-05T00:00:00Z","timestamp":1088985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> HIV vaccine testing in primates is an important method for determining the possibility of vaccine benefit in humans. Goals of HIV\u20101 vaccination include establishing neutralizing antibodies and a strong CD8<jats:sup>+<\/jats:sup> T\u2010cell response. We tested a novel vaccine conjugate for its ability to elicit relevant immune responses to HIV proteins and peptides in rhesus macaques. A neutralizing epitope, V3 loop peptide from HIV\u20101 envelope, was coupled to heat\u2010inactivated <jats:italic>Brucella abortus<\/jats:italic> (V3\u2010HKBA). Rhesus macaques were immunized with this conjugate in the anterior thigh. After two immunizations V3\u2010specific antibodies were found in the sera and at mucosal sites. Neutralizing activity of these antibodies was demonstrated by syncytia inhibition assays. Cellular immune recall responses were demonstrated by antigen\u2010specific induction of interferon\u2010gamma and Regulation on Activation Noraml T Cell Expressed and Secreted (RANTES) secretion <jats:italic>in vitro<\/jats:italic>. These results confirm and extend preliminary studies in mice that suggest HKBA is an effective carrier that promotes neutralizing antibody secretion at relevant mucosal sites, as well as cellular immune responses that are correlated with viral protection.<\/jats:p>","DOI":"10.1111\/j.1600-0684.2004.00068.x","type":"journal-article","created":{"date-parts":[[2004,7,5]],"date-time":"2004-07-05T19:10:13Z","timestamp":1089054613000},"page":"167-174","source":"Crossref","is-referenced-by-count":2,"title":["Systemic and mucosal immunity in rhesus macaques immunized with HIV\u20101 peptide and gp120 conjugated to <i>Brucella abortus<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Nancy","family":"Eller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Golding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Beining","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Inman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy E.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basil","family":"Golding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,5]]},"reference":[{"key":"e_1_2_21_2_2","unstructured":"UNAIDS:AIDS Epidemic Update. 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