{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:13:36Z","timestamp":1770981216097,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2005,8,10]],"date-time":"2005-08-10T00:00:00Z","timestamp":1123632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Current evidence suggests that a strong induced CD8 human immunodeficiency virus type 1 (HIV\u20101)\u2010specific cell mediated immune response may be an important aspect of an HIV vaccine. The response rates and the magnitude of the CTL responses induced by current DNA vaccines in humans need to be improved and cellular immune responses to DNA vaccines can be enhanced in mice by co\u2010delivering DNA plasmids expressing immune modulators. Two reported to work well in the mouse systems are interleukin (IL)\u201012 and CD40L. We sought to compare these molecular adjuvants in a primate model system. The cDNA for macaque IL\u201012 and CD40L were cloned into DNA vectors. Groups of cynomolgus macaques were immunized with 2\u2003mg of plasmid expressing SIVgag alone or in combination with either IL\u201012 or CD40L. CD40L did not appear to enhance the cellular immune response to SIVgag antigen. However, more robust results were observed in animals co\u2010injected with the IL\u201012 molecular adjuvant. The IL\u201012 expanded antigen\u2010specific IFN\u2010<jats:italic>\u03b3<\/jats:italic> positive effector cells as well as granzyme B production. The vaccine immune responses contained both a CD8 component as well a CD4 component. The adjuvanted DNA vaccines illustrate that IL\u201012 enhances a CD8 vaccine immune response, however, different cellular profiles.<\/jats:p>","DOI":"10.1111\/j.1600-0684.2005.00124.x","type":"journal-article","created":{"date-parts":[[2005,8,10]],"date-time":"2005-08-10T08:36:06Z","timestamp":1123662966000},"page":"262-270","source":"Crossref","is-referenced-by-count":82,"title":["SIV DNA vaccine co\u2010administered with IL\u201012 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques"],"prefix":"10.1111","volume":"34","author":[{"given":"Jean D.","family":"Boyer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara M.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele A.","family":"Kutzler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Parkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra A.","family":"Calarota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maninder K.","family":"Sidhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karuppiah","family":"Muthumani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Pavlakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Felber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Weiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,8,10]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7904381"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00244-2"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.050417697"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.6290"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/315229"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.11.8615-8623.1997"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.4.869"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.148.1.92"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i1.108"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.21.11715-11725.2004"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00546-4"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.14.6278-6286.2000"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.12.7180"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8097338"},{"key":"e_1_2_5_16_2","doi-asserted-by":"crossref","first-page":"4347","DOI":"10.4049\/jimmunol.155.9.4347","article-title":"IL\u20101 beta is required for IL\u201012 to induce production of IFN\u2010gamma by NK cells. A role for IL\u20101 beta in the T cell\u2010independent mechanism of resistance against intracellular pathogens","volume":"155","author":"Hunter CA","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4833-4840.2002"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.2.816"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0797-641"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(96)00260-5"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199803)28:03<1089::AID-IMMU1089>3.0.CO;2-L"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3986"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.3.827"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80226-0"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.68.7.4650-4655.1994"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.211"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.1.112"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.14.7490-7497.2004"},{"key":"e_1_2_5_29_2","doi-asserted-by":"crossref","first-page":"5071","DOI":"10.4049\/jimmunol.154.10.5071","article-title":"Dendritic cells produce IL\u201012 and direct the development of Th1 cells from naive CD4+ T cells","volume":"154","author":"Macatonia SE","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79869-0"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.4.1273"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040432"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.223"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19701-0"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.08.028"},{"key":"e_1_2_5_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4279"},{"key":"e_1_2_5_37_2","first-page":"722","article-title":"Modulation of immune responses to DNA vaccines by codelivery of cytokine genes","volume":"98","author":"Pan CH","year":"1999","journal-title":"J Formos Med Assoc"},{"key":"e_1_2_5_38_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.3.4.S119"},{"key":"e_1_2_5_39_2","doi-asserted-by":"crossref","first-page":"3495","DOI":"10.4049\/jimmunol.149.11.3495","article-title":"Natural killer (NK) cell stimulatory factor or IL\u201012 has differential effects on the proliferation of TCR\u2010alpha beta+, TCR\u2010gamma delta+ T lymphocytes, and NK cells","volume":"149","author":"Perussia B","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_5_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.69.9.5838-5842.1995"},{"key":"e_1_2_5_41_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.4.9.1483"},{"key":"e_1_2_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)93063-7"},{"key":"e_1_2_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0195-59"},{"key":"e_1_2_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(98)00179-5"},{"key":"e_1_2_5_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082305"},{"key":"e_1_2_5_46_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.68.6.793"},{"key":"e_1_2_5_47_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.2053"},{"key":"e_1_2_5_48_2","doi-asserted-by":"crossref","first-page":"2912","DOI":"10.4049\/jimmunol.162.5.2912","article-title":"IL\u201012 gene as a DNA vaccine adjuvant in a herpes mouse model: IL\u201012 enhances Th1\u2010type CD4+ T cell\u2010mediated protective immunity against herpes simplex virus\u20102 challenge","volume":"162","author":"Sin JI","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_5_49_2","doi-asserted-by":"publisher","DOI":"10.1089\/104303401750214302"},{"key":"e_1_2_5_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301274"},{"key":"e_1_2_5_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404739101"},{"key":"e_1_2_5_52_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1083317"},{"key":"e_1_2_5_53_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2002.01087.x"},{"key":"e_1_2_5_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60387-9"},{"key":"e_1_2_5_55_2","doi-asserted-by":"publisher","DOI":"10.1089\/0889222041725253"},{"key":"e_1_2_5_56_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200008180-00010"},{"key":"e_1_2_5_57_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250307"}],"container-title":["Journal of Medical Primatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0684.2005.00124.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0684.2005.00124.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T18:42:28Z","timestamp":1693507348000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0684.2005.00124.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,10]]},"references-count":56,"journal-issue":{"issue":"5-6","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["10.1111\/j.1600-0684.2005.00124.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0684.2005.00124.x","archive":["Portico"],"relation":{},"ISSN":["0047-2565","1600-0684"],"issn-type":[{"value":"0047-2565","type":"print"},{"value":"1600-0684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8,10]]}}}